Genetic polymorphism in the POU1F1 gene in Kalahari Red and two Nigerian goat breeds and their relationship with litter size

Authors

  • Babatunde Shittu Olasege Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, PMB 2240 Abeokuta, Nigeria; / School of Chemistry and Molecular Biosciences, Faculty of Science, The University of Queensland, Brisbane, Queensland, Australia
  • Martha Nchang Bemji Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, PMB 2240 Abeokuta, Nigeria
  • Eveline Mengwi Ibeagha -Awemu Agriculture and Agri-Food Canada, Sherbrooke Research and Development Center, Sherbrooke, Quebec, Canada
  • Mani Isa Adamu Key Laboratory of Animal Genetics, Breeding and Reproduction, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, PR, Beijing, China;/Department of Animal Science, Usmanu Danfodiyo University, Sokoto, Nigeria
  • Wheto Mathew Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, PMB 2240 Abeokuta, Nigeri
  • Ganiu Damilare Sulaimon Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, PMB 2240 Abeokuta, Nigeria
  • Musa Olarenwaju Ayinde Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, PMB 2240 Abeokuta, Nigeria
  • Basirat Olasumbo Sodimu Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, PMB 2240 Abeokuta, Nigeria
  • Damilola Abimbola Ogunniyi Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, PMB 2240 Abeokuta, Nigeria
  • Ngozi Okwelum Institute of Food Security, Environmental Resources and Agricultural Research, Federal University of Agriculture, Abeokuta, Nigeria
  • Bamidele O Oluwatosi Institute of Food Security, Environmental Resources and Agricultural Research, Federal University of Agriculture, Abeokuta, Nigeria

DOI:

https://doi.org/10.46325/gabj.v5i1.166

Keywords:

POU1F1 gene, mutation, West African Dwarf, Red Sokoto, Kalahari Re

Abstract

POU1F1 gene controls cell differentiation and animal growth by binding to target DNA promoter sequence, thereby auto-regulating its own expression and expression of growth hormone (GH), prolactin (PRL) and thyroid-stimulating hormone beta sub-unit (TSHβ) genes. Therefore, the exploration of caprine POU1F1 gene polymorphisms may be vital in the formulation of conservation and breed improvement strategies.  In this study, POU1F1 gene was characterized for sequence polymorphisms in 366 individuals from two Nigerian goat breeds ((West African Dwarf (WAD) and Red Sokoto (RS)) and one South African goat breed (Kalahari (KR)). The effects of polymorphisms on litter size were investigated using linear mixed model. Two intronic mutations (g.306G>A and g.11236C>T) were identified. However, no significant association was found between the Single Nucleotide Polymorphisms (SNPs) and litter size in the three populations. The genetic distance based on POU1F1 investigated region revealed that the two Nigerian breeds and the South African breed were identical (pairwise genetic distance of 0.00). Phylogenetic tree constructed from the pairwise distance clustered the three breeds into a single clade with the two Nigerian goat breeds having a more recent common ancestor. Structural analysis of the POU1F1 protein confirmed that Pit-Oct-Unc transcription factors domain (POU) and Homeodomain (HOX) domains are conserved in mammals, with several overlapping sub-domains across the same region in all the three populations. We found a subdomain Subfamily of SANT domain or myb/SANT-like domain in Adf-1 (MADF) in goat, cattle, buffalo and camel that has not been reported in mammals

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Published

01/25/2021

How to Cite

Olasege, B. S. ., Bemji, M. N. ., Ibeagha -Awemu, E. M., Adamu , M. I., Mathew , W., Sulaimon, . G. D. ., Ayinde, M. O. ., Sodimu, B. O. ., Ogunniyi, D. A. ., Okwelum, N. ., & Oluwatosi, B. O. . (2021). Genetic polymorphism in the POU1F1 gene in Kalahari Red and two Nigerian goat breeds and their relationship with litter size. Genetics & Biodiversity Journal, 5(1), 42–59. https://doi.org/10.46325/gabj.v5i1.166

Issue

Vol. 5 No. 1 (2021): v5i12021

Section

Original Article

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.