Mycoflora of cork after exploitation and storage in a cork oak forest of the West North of Algeria
Keywords:
Cork, fungal flora, cork plantations, cork oak stands, West Algeria.Abstract
Introduction: The present work, concerns the investigation of the mycoflora of cork stored in M'sila’s forest (western Algeria) to dry before using in industry.
Methods: 15 planks of cork taken at random from different parts of the storage piles were analyzed before and after boiling. Fungi identification based on morphological and molecular (rDNA) characteristics
Results: An initial list of 31 species belonging to several systematic genera were isolated. The most important, appearing in cork before and after treatment, belong to the genera Penicillium, Aspergillus, Alternaria, Cladosporium and the Mucorales order. Some species belonging to these genera have already proved their ability to produce volatile components such as TCA in cork and cork stoppers in other studies around the world. Other species, however, have only appeared in unboiled cork, such as Trichoderma sp, Fusarium sp, Gelasinospora brevispora, Sordaria humana, and two basidiomycetes indicative of high humidity and poor cork storage conditions, Coprinus xanthothrix and Schizopphyllum commune.
Conclusion: The natural cork stoppers industry requires the use of healthy cork boards of a good mycological quality to avoid denaturing corked food products.
References
- Alvarez-Rodriguez M.L, Belloch C, Villa M, Uruburu F, Larriba G, Coque JJR (2003). Degradation of vanillic acid and production of guaiacol by microorganisms isolated from cork samples. FEMS Microbiology Letters 220: 49-55. https://doi.org/10.1016/S0378-1097(03)00053-3.
- Alvarez-Rodriguez ML, Lopez-Ocana L, Lopez-Coronado JM, Rodriguez E, Martinez MJ, Larriba G, Coque JJR (2002). Cork taint of wines: role of the lamentous fungi isolated from cork in the formation of 2,4,6-trichloroanisole by O-methylation of 2,4,6-trichlorophenol. Appl. Environ. Microbiol. 68: 5860. https://doi.org/10.1128/AEM.68.12.5860-5869.2002.
- Amon JM, Vandepeer JM, Simpson RF (1989). Aust NZ Wine Ind J 4:62-69.
- Barnett HL, Hunter BB (1998). Illustrated Genera of Imperfect Fungi. 4th ed, Burgess Publishing Company, Minnesota, USA, 223 p.
- Basilio MC, Tenreiro R, Crespo T, San Romao MV (2007). Fungi on wine works. Food Mycology. 64p.
- Botton B, Breton M, Gautier S, Guy PH, Larpent JP, Reymond P, Sanglier YJJ, Veau P (1990). Moisissures utiles et nuisibles importance industrielle. 2ème édition. Collection biotechnologies, Masson. 512p.
- Boudaoud N (2006). Potentialités d’utilisation du nez électronique à spectromètre de masse pour la caractérisation de la fraction volatile du liège œnologique. Thèse Doc. Chimie analytique. Institut National Agronomique Paris-Grignon, Ecole Doctorale ABIES.
- Butzke C, Evans TJ, Ebeler SE (1998). In: Waterhouse AL, Ebeler SE (eds) Chemistry of wine flavour, ACS symposium series 714. American Chemical Society, Washington, DC, pp 208-216.
- Butzke CE, Evans TJ, Ebeler SE (1999). Détection de goût de bouchon dans le vin en utilisant microextraction en phase solide automatisée en combinaison avec GC/MS-SIM. ACS Symp. Ser. 714: 208-216.
- Caldentey P, Maria DF, Mazzolent V, Careri M (1998). Volatile Compounds Produced by microorganisms isolated from cork. Flavour Frager J. 13:185-188.
- Calvo MA, Agut M, Larrondo J, Esteban C, Codina J (1995). Identication of fungal hyphae and conidia in samples of altered cork. Microbios 81: 41-44.
- Calvo MA, Agut M (1996). Microbiological aspects of cork. In: Proceedings of the Second International Symposium on Cork, pp. 33-36.
- Ellis MB (1971). Dematiaceous Hyphomycetes. CAB, England, p. 608.
- Ellis MB (1976). More Dematiaceous Hyphomycetes. CAB, England, p.507.
- Faustino A, Pires RC, Marum L (2023). Periderm differentiation: a cellular and molecular approach to cork oak. Trees, 37(3), 627-639. https://doi.org/10.1007/s00468-023-02398-1
- Fuller P (1995). Closing in on an Industry Problem. Aust. N. Z. Wine Ind. J 10:58-60.
- Hawksworth DL, Kirk PM, Sutton BC, Pegler DN (1995). Ainswoorth & Bisby’s Dictionary of the Fungi. 8th ed., CAB International, UK, 616p.
- Jensen N, Varelis P, Whiteld FB (2001). Formation of guaiacol in chocolate milk by the psychrotrophic bacterium Rahnella aquatilis. Lett. Appl. Microbiol. 33: 339-343.
- Kiffer E, Morelet M (1997). Les Deutèromycetes – classification et clés d’identification générique. INRA Editions, Paris, 306 pp.
- Lanier GN, Burns BW (1978). Barometric flux. Effects on the responsiveness of bark beetles to aggregation attractants. J. Chem. Ecol. 4 : 139-47.
- Lee TH, Simpson RF (1993). "Goût de microbiologie et de chimie de liège". In Fleet GH" Microbiologie du vin et de la biotechnologie." 1ère édition: Chur (USA): Hardwood Academic Publishers. Chapitre 12 : 353-372.
- Liacopoulos D, Barker D, Howland PR, Alcorso DC, Pollnitz AP, Skouroumounis GK, Pardon KH, McLean HJ, Gawel R, Sefton MA (1999). In: Blair RJ, Sas AN, Hayes PF, Høj PB (eds) Proceedings of the 10th Australian wine industry technical conference, Sydney, pp 224–226.
- Maga JA (1978). Simple phenol and phenolic compound in food flavours. Crit. rev. Sc. Food 10: 323-372.
- Oliveira AC, Peres CM, Correira Pires JM, Silva Pereira C, Vitorino S, Figueiredo Marques JJ, Barreto Crespo MT, San Romao MV (2003). Cork stoppers industry: defining appropriate mould colonization. Microbiol. Res. 158: 117-124. https://doi.org/ 10.1078/0944-5013-00183
- Orr RV, Shewfelt RL, Huang CJ, Tefera S, Beuchat LR (2000). Detection of guaiacol produced by Alicyclobacillus acidoterrestris in apple juice by sensory and chromatographic analyses and comparison with spore and vegetative cell populations. J. Food Prot. 63: 1517-1522. https://doi.org/ 10.4315/0362-028x-63.11.1517
- Pereira H, Taco C, Lopes F (2003). La variation dans l'arbre de l'épaisseur du liège et du dos des planches de liège pour des chênes-lièges en pleine production. Anais do Instituto Superior de Agronomia, 209-221.
- Pettipher GL, Osmundson ME, Murphy JM (1997). Methods for the detection and enumeration of Alicyclobacillus acidoterrestris and investigation of growth and production of taint in fruit juice and fruit-juice containing drinks. Lett. Appl. Microbiol. 24: 185-189. https://doi.org/ 10.1046/j.1472-765x.1997.00373.x
- Rakotonirainy, MS (2013). Viabilité des moisissures dans les taches de foxing/dosage de l'ATP par bioluminescence. In La science de conservation du patrimoine et le développement durable. Acquis, Recherche, Innovation. hal-02947973, version 1
- Samson RA, Hoekstra ES, Frisvad, JC, 2004. Introduction to Food- and Airborne Fungi. 7th edition. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands.
- Samson RA, Houbraken J, Thorane U, Frisvad JC, Andersen B (2009). Food and Indoor Fungi. CBS-KNAW Fungal Biodiversity Centre Utrecht, The Netherlands. 356p
- Silva Pereira C, Figueiredo Marques JJ, Romao MV (2000). Cork taint in wine: Science knowledge and public perception. A critical review. Crit. Rev. Microbiol. 26:147-162. https://doi.org/10.1080/10408410008984174
- Silva Pereira C, Pires A, Valle MJ, Vilas Boas L, Figueiredo Marques JJ, San romao MV (2000b). Role of the Chrysonilia sitophila in the quality of cork stoppers for sealing wine bottles. J. Ind. Microbiol. Biotechnol. 24: 256-261. https://doi.org/10.1038/sj.jim.2900815
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011). MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731-2739. https://doi.org/10.1093/molbev/msr121
- Tanner H, Zanier C, Buser HR (1981). Schweiz Z Obst Weinbau 117:97–103.
- Vlachos P, Kampioti A, Konaros M, Lyberatos G (2007). Developement and evaluation of alternative processes for sterilization and deodorization of cork barks and natural cork stoppers. Eur Food Res Technol. 225:653-663. https://doi.org/10.1007/s00217-006-0461-3.