Formulation of vitamin D3 nanoemulsion as a new nanonutraceutical
Keywords:
Cholecalciferol-Ethyl oleate- Nanoceutical- Nanoemulsion- Ultraturrax.Abstract
Introduction : Hypovitaminosis D is a major global health problem that may be implicated in many diseases. Unfortunately, the current oral treatments don’t provide sufficient efficacy due to low vitamin D bioavailability and weak stability. In order to improve these essential parameters, we formulate a nanoemulsion with the required specifications as a nanoceutical form.
Methods: Vitamin D3 nanoemulsion was prepared using high shear homogenisation method at 6500 rpm for 10 minutes. Ethyl Oleate was chosen as an oily vehicle at a proportion of 1% in the formulation. Tween 20 and Labrasol were selected as stabilizers (Smix ratio 3:10 respectively). The formulation was characterised by globule size, dispersion index (PDI), transmittance, refractive index, pH, conductivity, viscosity, physical stability, zeta potential, dilution robustness and their diffusion profiles through dialysis bags (MWCO 8000-14000).
Results : A transparent Vitamin D nanoemulsion was obtained with ethyl oleate, stabilizers (Labrasol, Tween 20) and water at ratio 1:9:90. The tenuity of the globule sizes showed 12.4 nm of mean diameter and 0.299 of PDI. The refractive index and transmittance were respectively 1.35 and 91 %. The system presents a very good stability to centrifugation test, freeze thaw cycles and sufficient robustness to high dilutions. The in vitro diffusion profile of nanoemulsion showed concentrations higher 200 times than those of the vitamin D oily solution.
Conclusion: This experience highlighted the great interest of nanoemulsion as an effective vehicle for improving the stability and the in vitro membrane permeability of vitamin D in the gastric environment.
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