International Journal of Nutrition and Biotechnology Advancements
The international journal « Nutrition and Biotechnology Advancements » is a peer-reviewed, interdisciplinary open access journal with an expanded scope that not only includes innovations in basic and applied life sciences, but also manuscripts at the intersection of traditionally defined disciplines, i.e., cross-disciplinary studies, where integrative and emerging fields of investigation are being formed. The expanded scope of JNBA encopasses a broad range of topics within the field of global nutritional sciences but is not limited to the following: Nutrition, physiology and metabolism, nutrients, nutraceuticals and immune system, biotechnology advances and biomimetics, agriculture, bioresources and agro-materials, molecular modeling and reactivity. The journal welcomes original research articles, systematic reviews, meta-analyses, case studies, opinion pieces that achieve the advancement of food biotechnology and nutrition sciences and other types of contributions that advance the understanding of nutritional and biotechnology issues. Manuscripts should prove strong research methodology, clear interpretations of results, and healthy nutrition & biotechnology applications. Publisher: University Abou Bekr Belkaïd, Tlemcen, Algeria. Online ISSN: 2992-202X.
The potential impact of plant based nutrition on the metabolic syndrome and overall health: a narrative review
Background: This review article aims to highlight the beneficial effects of plant-based nutrition as a safe, effective and sustainable nutritional intervention in the face of the increasing global prevalence of metabolic syndrome, which is characterized by a cluster of risk factors such as insulin resistance, obesity, dyslipidaemia and hypertension. Materials and Methods: In order to gain a comprehensive understanding of the topic, a literature search was conducted using various databases and keywords related to plant based nutrition, overall health, metabolic syndrome, metabolic health. The search was limited to peer-reviewed articles published in English between 2006 and 2025. Animal model studies were excluded to focus on human research. The criteria for inclusion in this review were that studies examined the relationship between plant based nutrition and metabolic syndrome, were published in a peer-reviewed journal, and were written in English. After careful consideration, a total of 24 studies were selected for inclusion. Results : A substantial body of literature addressing the impact of plant-based nutrition on metabolic syndrome underscores the capacity of plant-derived bioactive compounds to enhance markers of inflammation, insulin sensitivity, and lipid profiles, concurrently reducing oxidative stress. This finding suggests that a plant-based diet may have a beneficial effect on overall health. Conclusion: Studies on this topic have shown that plant-based diets rich in dietary fibers, phytonutrients, and antioxidants significantly reduce systemic inflammation and improve metabolic parameters associated with metabolic syndrome. Synthesizing both epidemiological and clinical trial data, it is emphasized that adherence to a plant-based diet is associated with improved metabolic profiles and a reduced risk of developing metabolic syndrome.
Copyright (c) 2025 International Journal of Nutrition and Biotechnology Advancements
The potential impact of probiotics on overall health and well-being : A narrative review
Introduction: This review article endeavors to shed light on the current findings regarding the impact of probiotics on metabolic processes, their role in the gut-brain axis, and their broader implications for overall well-being. It also underscores the significance of maintaining a balanced diet, which includes probiotic-rich foods, as a key component of a healthy lifestyle. Methods: In order to gain a comprehensive understanding of the topic, a literature search was conducted using various databases and keywords related to probiotics, overall health, well-being, and gut microbiota. The search was limited to peer-reviewed articles published in English between 2005 and 2025. Animal model studies were excluded to focus on human research. The criteria for inclusion in this review were that studies examined the relationship between probiotics and overall health, were published in a peer-reviewed journal, and were written in English. After careful consideration, a total of 24 studies were selected for inclusion. Results: The potential for probiotics to influence the balance of intestinal bacteria and, by extension, contribute to human health, is a promising area of research. It is important to note that a healthy balance of bacteria can be impacted by a variety of factors, such as diet, stress, antibiotic use, and certain diseases. It is possible that probiotics could help restore this balance by competing with pathogens for nutrients and adhesion sites in the intestine. Conclusion: Research on the health effects of probiotics can help us understand their effects on human health. This research may pave the way for new treatments, probiotic formulations, and interventions to address health issues. As the field develops, probiotics may hold promise for health maintenance and the prevention and management of complex diseases.
Formulation of vitamin D3 nanoemulsion as a new nanonutraceutical
Introduction : Hypovitaminosis D is a major global health problem that may be implicated in many diseases. Unfortunately, the current oral treatments don’t provide sufficient efficacy due to low vitamin D bioavailability and weak stability. In order to improve these essential parameters, we formulate a nanoemulsion with the required specifications as a nanoceutical form. Methods: Vitamin D3 nanoemulsion was prepared using high shear homogenisation method at 6500 rpm for 10 minutes. Ethyl Oleate was chosen as an oily vehicle at a proportion of 1% in the formulation. Tween 20 and Labrasol were selected as stabilizers (Smix ratio 3:10 respectively). The formulation was characterised by globule size, dispersion index (PDI), transmittance, refractive index, pH, conductivity, viscosity, physical stability, zeta potential, dilution robustness and their diffusion profiles through dialysis bags (MWCO 8000-14000). Results : A transparent Vitamin D nanoemulsion was obtained with ethyl oleate, stabilizers (Labrasol, Tween 20) and water at ratio 1:9:90. The tenuity of the globule sizes showed 12.4 nm of mean diameter and 0.299 of PDI. The refractive index and transmittance were respectively 1.35 and 91 %. The system presents a very good stability to centrifugation test, freeze thaw cycles and sufficient robustness to high dilutions. The in vitro diffusion profile of nanoemulsion showed concentrations higher 200 times than those of the vitamin D oily solution. Conclusion: This experience highlighted the great interest of nanoemulsion as an effective vehicle for improving the stability and the in vitro membrane permeability of vitamin D in the gastric environment.
Evaluation physicochemical stability of metronidazole gel 1 %formulation
Introduction : The stability of topical formulations is critical for their efficacy and safety. This study aimed to formulate a 1% metronidazole gel for treating rosacea and to evaluate its physicochemical stability. Methods: This study formulated gels using Carbopol 935 as the gelling agent. The prepared samples contained metronidazole on its own or supplemented with either methylparaben or EDTA as a preservative. To assess stability, the formulations were stored for 30 days under two conditions : room temperature and refrigeration. Their stability was evaluated through organoleptic inspection, alongside monitoring of pH and conductivity, and analysis via Fourier- Transform Infrared (FTIR) spectroscopy employing an Attenuated Total Reflection (ATR) accessory. Results: Macroscopic analysis revealed no changes in the gels' homogeneity or color over time. Measurements of pH and conductivity showed no significant differences (p>0.05) for most formulations, except for a predictable pH decrease in the EDTA-containing gel. Critically, FTIR analysis confirmed the absence of chemical interactions. The characteristic metronidazole peaks at ~1636 cm⁻¹ (C=C, C=N stretching) and ~3313 cm⁻¹ (C-H stretching) remained unchanged in position and intensity in all formulations after 30 days, with no appearance of new spectral bands. The results from all analytical techniques demonstrate the stability of the 1% metronidazole gel. The consistency of the FTIR spectra confirms the absence of chemical degradation or incompatibility between metronidazole, Carbopol 935, and the preservatives. It is concluded that Carbopol 935 is a suitable polymer for formulating a stable topical metronidazole gel, which remains chemically stable for at least 30 days, even at room temperature.
From Traditional Use to Toxicology: Ethnobotanical Analysis and Acute Toxicity Assessment of Berberis sp in Algeria
Introduction: This study aims to bridge the gap between traditional knowledge and toxicological assessment, focusing on species of the Berberis genus used in Algerian folk remedies. Materials and Methods: An ethnobotanical study was conducted based on a bibliographic analysis and field surveys. At the same time, experimental test was conducted to verify the immediate harmful effects on BALB/c mice using an aqueous extract obtained by boiling 10% of the root bark of wich has being identified as Berberi shispanica (later known as Berberis vulgaris ssp. australis), collected in Tikejda forest arean (Djurdura national park). Experimental test has being conducted in accordance with OECD 423 guidelines. Results: The ethnobotanical survey revealed frequent confusions between Berberis vulgaris and Berberis hispanica, we fund a marked lack of awarness ignorance among 93% of the herbalists interviewed about the availability and existence of Berberis taxa in Algeria, as well as the origin of plant material sold. This ignorance increases the risk of misidentification. The LD50 was estimated at 3162.27 mg/k, while the total lethal dose was 5000 mg/kg in males and 6000 mg/kg in females, indicating a higher toxicological sensitivity in males.
The Effects of Weight Control Management on Metabolic Health: A Mini Review
Introduction: This study aims to synthesize findings from research studies and review articles in this field to provide an in-depth analysis of the multifaceted effects of weight control management on metabolic processes by synthesizing evidence from nutritional research, physiology studies, and clinical research. Methods: In order to gain a comprehensive understanding of the topic, a literature search was conducted using various databases and keywords related to physical exercise, metabolic health, quality of life, weight control management, obesity. The search was limited to peer-reviewed articles published in English between 1998 and 2024. Animal model studies were excluded to focus on human research. The criteria for inclusion in this review were that studies examined the relationship between weight control management and metabolic health, were published in a peer-reviewed journal, and were written in English. After careful consideration, a total of 24 studies were selected for inclusion. Results: It has been suggested that a combination of a nutritionally balanced diet and regular physical activity may have a synergistic effect and lead to more successful results in weight management. This integrated approach holds promise in achieving a desired weight and promoting a sustainable lifestyle, which has been shown to contribute to the prevention of chronic diseases such as obesity, diabetes and heart disease. It should be emphasized that individuals can develop a balanced and healthy lifestyle by prioritizing these two pillars - nutrition and exercise. Conclusion: The path to effective weight control is paved with consistent healthy eating and regular physical activity. Adopting this comprehensive strategy may facilitate the attainment of weight objectives, enhance overall health and well-being, and promote a sense of vitality. A comprehensive diet and exercise program has been demonstrated to be a strategy for achieving and maintaining an ideal weight, ensuring long-term health benefits and enhancing quality of life.
Ciguatera on the Tlemcen Coast (Algeria): Survey and Preliminary Data
Introduction: Oceans are a major source of food, but pollution and coral reef degradation encourage the proliferation of toxic microalgae such as Gambierdiscus spp., which are responsible for the production of ciguatoxins. These toxins accumulate along the food chain, causing food poisoning in humans known as ciguatera, mainly after consuming predatory fish(e.g.,grouper,barracuda). Although traditionally tropical, this illness poses an increasing threat in the Mediterranean due to climate change. Methods: The Eurocigua project (2016–2021) confirmed the presence of these microalgae in certain Mediterranean regions, but no cases have been reported in Algeria. Results: A local study conducted in the Tlemcen province revealed an almost complete lack of knowledge about the disease and no reported cases. However, rising temperatures and the importation of fish from high-risk areas could promote the future emergence of ciguatera in Algeria. Conclusion: The absence of a standardized method for detecting ciguatoxins complicates monitoring. International examples (e.g. Canary Islands) demonstrate the effectiveness of systems combining chemical analyses with epidemiological surveillance.
Kinetic study of major components of leaves’ essential oil from Dettrichia viscosa (L.)
Introduction: The Algerian Dettrichia viscosa essential oil yield evolution during water distillation has been investigated as a function of grinding leaves. Methods: GC/MS analysis helped to determine the extraction kinetics of the major components of this essential oil. Results: The results show that there are two kinetic types. We have tried to correlate this kinetic type with the essential oil location in the vegetable material and chemical composition. The microscopic observations show that most of the essential oil is localized in parenchyma but there are some in glandular hairs. Conclusion: The water distillation of grinding leaves offers important advantages over the whole leaves: shorter isolation times (50 min) each type of kinetics corresponds to the compound considered localized in endogenous or exogenous deposits.
Theoretical study of chlorpromazine Drug and its metabolites: DFT calculation and QSAR toolbox investigation
Introduction: Pharmaceutical compounds are among the most important products for the treatment and prevention of various problems. However, their excess or incompatibility with other compounds can harm human health. These compounds include, in particular, psychological and neurological effects, which have recently become widely used to treat schizophrenia and behavioraldisorders. Methods: This work aims to study chlorpromazine, a pharmaceutical compound used as an antipsychotic in psychological and neurological conditions, as well as its characteristics, chemical and biological properties, and interactions with other compounds. The drug and its metabolites induce the human body to avoid them, as well as the resulting hot flashes. This theoretical study was carried out using molecular modelling with Gaussian program. Density functional theory (DFT) was used to identify the geometric, energetic, and reactivity properties of the systems studied. To study the biological activity and toxicity of our compounds, we used the QSAR-Toxtree program. Results: Our results allowed us to determine the most biologically active compound and conclude that the resulting compounds are likely to produce more or less toxic compounds. In light of these results, it can be stated that: the compound chlorpromazine is more biologically stable compared to its metabolites, which confirms that it is an active substance in medical applications. In general, we found that all the compounds studied have chemical and biological activity and are combined with each other in the resulting potential toxicity.
Assessment of the iodine content of salt consumed in the town of Bechar
Introduction: Evaluating the iodine content in household salt is crucial for public health, particularly in regions where legislation mandates iodization. In Bechar, Algeria, thirty years after the government established regulations for salt iodization at 30-50 ppm, it is essential to assess compliance and effectiveness. This study aims to evaluate the iodine content in salt consumed by households in Bechar and its alignment with national standards. Methods: A cross-sectional survey was conducted involving 509 households in Bechar. Schoolchildren were randomly selected from a comprehensive list and asked to provide a 50g sample of salt consumed by their families. The iodine content of these samples was then measured and analyzed against national recommendations. Results: The median iodine content in the salt samples was 17.50 mg/kg, below the recommended 30-50 ppm. Over 80% of households used either inadequately or excessively iodized salt. Specifically, 73.67% of the samples had iodine levels below 30 ppm, while 7.07% exceeded 50 ppm. Only 19.25% of the samples met the recommended standards. The most commonly consumed salt brands in this study are private label brands. The majority of the samples analyzed show iodine levels below 30 ppm, which is below the recommended level. Conclusion: This study reveals a significant shortfall in the adequate iodization of household salt in Bechar compared to Algerian standards. Identifying and addressing the causes of this deficiency is critical to ensuring consumer protection and improving public health outcomes.
The impact of taurine on healthy nutrition and Overall health
Background: This review article aims to provide a comprehensive overview of the diverse effects of taurine on human health. It seeks to synthesize the existing research findings and explore the potential therapeutic applications of taurine in address ing various medical conditions. Materials and Methods: A comprehensive search of the literature was conducted using various databases and a range of keywords, including "taurine," "nutrition," "health," "metabolism," "physical activity," and "overall health." It was intended to ensure that the search was as inclusive as possible, and therefore several different terms have been included. The review included a variety of study types, such as randomized controlled trials, cohort studies, and systematic reviews , to ensure a comprehensive and diverse range of evidence, in line with the best practice for such a review. To ensure a comprehensive and diverse range of evidence, the inclusion criteria for this review were established to filter studies that assess the relationship among "taurine," "nutrition," "health," "metabolism," "physical activity," and "overall health." After careful consideration, a total of 26 studies were selected for inclusion in this review. This review aims to shed light on the relationship among eating disorders, wellness, physical activity, and overall health. To ensure the quality and relevance of the selected studies, we limited the search to peer-reviewed articles published in English between 2001 and 2024. Results: Taurine has the potential to offer a range of benefits for athletes, both in terms of supporting their performance and promoting overall health and well-being. While its effects on endurance and strength performance may vary from one individual to another, and there is much to be learned about how it can be most effectively used, the antioxidant properties, hydration benefits, and positive impacts on muscle recovery and cardiovascular health remain consistent across different athletic populations. Conclusion: There is considerable potential for taurine to play a role in nutritional and therapeutic strategies targeting diabetes, cardiovascular diseases, and oxidative stress -related conditions. Given its diverse beneficial properties, it seems prudent to include taurine in public health discussions and clinical practice. There is a clear need for continued exploration to fully harness taurine's capabilities in promoting holistic health.
Copyright (c) 2024 International Journal of Nutrition and Biotechnology Advancements
Phenotypic and genotypic insights into a halotolerant bacterium with antimicrobial potential from El Golea Lake
Introduction: The antimicrobial potential of a halotolerant bacterial strain, LMB3982, isolated from the hypersaline Lake El Golea in Algeria at 25% NaCl (w/v), was investigated. The study aimed to evaluate the antimicrobial activity of this strain and to characterize its phenotypic and genotypic properties, with a focus on its bioactive compounds and environmental adaptability. Methods: The strain was subjected to primary and secondary screenings for antimicrobial activity against a panel of test microorganisms. The influence of growth medium and incubation time on the production of antimicrobial metabolites was assessed. Various organic solvents were used to extract antimicrobial compounds, which were then evaluated for their efficacy. Thin-layer chromatography (TLC) was performed on chloroformic extracts using three solvent systems, and chemical properties were analyzed using infrared (IR) s pectroscopy. Additionally, a taxonomic study based on phenotypic and genotypic identification, including 16S rRNA gene sequencing, was conducted. Results: Strain LMB3982 demonstrated significant antimicrobial activity against most test microorganisms, with maximum activity observed after 72 hours of culture on nutrient agar. Chloroform was identified as the most effective solvent for extracting bioactive compounds. TLC analysis revealed the presence of two types of molecules: amines and phenols. IR spectros copy confirmed these findings, showing characteristic peaks corresponding to these compounds. Biochemical tests indicated that the strain could produce various enzymes, utilize multiple sugars, and grow under a wide range of conditions: NaCl concentrations of 0–25%, pH levels of 5–11, and temperatures of 15–37°C. Phylogenetic analysis of the 16S rRNA gene sequence positioned strain LMB3982 as a novel taxon within the Bacillales order, showing 96% similarity to Bacillus pseudofirmus and 93.3% to Bacillus halodurans Conclusion:. These findings highlight the potential of strain LMB3982 for biotechnological applications, particularly in the production of novel antimicrobial compounds and enzymes adapted to extreme conditions.
Pathogenicity of Biscogniauxia mediterranea (De. Not. Kunze.) to Quercus seedlings
Introduction: The fungus Biscogniauxia mediterranea (syn.: Hypoxylon mediterraneum) is one of the most dreaded pathogens on trees of the genus Quercus throughout the Mediterranean basin.The present work aims to study in vivo the pathogenicity of two isolates of B. mediterranea from symptomatic trees of the cork oak of the two forests of western Algeria. Methods: The pathogenicity of the isolates was tested on young seedlings of Q. suber and Q. ilex aged one year, by inoculation method, under controlled conditions (T° ~ 19 to 24 °C). Results: After 30 days of inoculation, the two isolates confirmed their pathogenicity towards cork oak and holm oak seedlings causing necrotic lesions on the stem reaching up to 45 mm in certain seedlings. Drying and chlorosis of the leaves are also observed. Conclusion: Given the potential threat posed by the inherent aggressiveness of Biscogniauxia species, further research is deemed necessary to curb the development of charcoal disease on oak trees.
The effect of sports and energy drink consumption on overall health and wellness
Background: The aim of this study is to shed light on the physiological and metabolic effects of sports drinks and energy drinks, which are of great interest not only to sports scientists and nutritionists, but also to medical professionals with an interest in their public health implications. Materials and Methods: A comprehensive search of the literature was conducted using various databases and a range of keywords, including "sports drink", "energy drink", "physical activity", "active life", and " overall health ". It's intended to ensure that the search was as inclusive as possible, and we therefore included several different terms. The review encompassed a multitude of study types, including randomized controlled trials, cohort studies, and systematic reviews, with the objective of ensuring a comprehensive and diverse range of evidence, in accordance with the established best practices for such a review. To ensure a comprehensive and diverse range of evidence, the inclusion criteria for this review were established to filter studies that assess the relationship between "sports drink," "energy drink," "physical activity," "active life," and "overall health." The objective was to gain the most insight possible, and a total of 25 studies were selected for inclusion in this review. This review is concerned with the relationship between sports drinks, energy drinks, physical activity, and overall health. In order to guarantee the quality and relevance of the selected studies, the search was restricted to peer-reviewed articles published in English between 2006 and 2024. Results: It would be beneficial to consider the data resulting from the investigation of the phy siological and metabolic effects of sports and energy drinks, with a view to encouraging informed decision -making among consumers of these products and sports and health scientists. It would be beneficial to the extent that the scientific basis of these drinks can be explained from metabolic and physiological perspectives, to reveal the contribution of these products to a wider awareness of their roles in nutrition, performance enhancement and public health. Conclusion: Sports and energy drinks have become a common feature of today9s fitness and active lifestyle culture. Promoted to improve athletic performance and energy, these sports drinks are sought after by a variety of individuals, including those at the peak of their profession as professional athlete s. Given that they are designed to provide a quick energy boost for the public and athletes, it seems reasonable to suggest that this product would undergo extensive physiological and metabolic analysis concerning both athletic performance and wellness.
Flaxseed oil supplementation improves lipid levels in the liver of aged obese rats
Introduction: Flaxseed (Linum usitatissimum L.) oil is an excellent functional oil containing various unsaturated fatty acids, mainly composed of linolenic acid, which is believed to have a variety of beneficial physiological and functional properties. This study was conducted to evaluate the hepato protective effects of flaxseed oil. The aim was to study how dietary linseed oil modulates lipid level in the liver of aged obese rats. Methods: aged rats were fed a control (C) or a Cafeteria (CAF) diet enriched or non-enriched with linseed oil at 5%. Changes in cholesterol (TC), triglyceride (TG) and LCAT activity levels in the liver were determined at the end of the experiment. Results: The cafeteria diet led to higher weight liver and hyperlipidemia, contents in liver of aged obese rats. Linseed oil (LO) supplementation modulates liver tissue TC and TG contents in both control and obese aged rats , with beneficial effects resulting in lower liver weight, decreased in TC,TG, low density lipoprotein-high density lipoprotein1(LDL-HDL-C), and increased in serum LCAT activity Conclusion: The supplement of linseed oil in the diet of aged obese rats is effective in amelioration of [1]lipid liver profile in aged rats, which might contribute to prevent obesity and dyslipidemia. * Corresponding Author : Laissouf Ahlem; email. ahlemlmd15@yahoo.fr
Copyright (c) 2024 Journal of Nutrition and Biotechnology Advancements
Mycoflora of cork after exploitation and storage in a cork oak forest of the West North of Algeria
Introduction: The present work, concerns the investigation of the mycoflora of cork stored in M'sila’s forest (western Algeria) to dry before using in industry. Methods: 15 planks of cork taken at random from different parts of the storage piles were analyzed before and after boiling. Fungi identification based on morphological and molecular (rDNA) characteristics Results: An initial list of 31 species belonging to several systematic genera were isolated. The most important, appearing in cork before and after treatment, belong to the genera Penicillium, Aspergillus, Alternaria, Cladosporium and the Mucorales order. Some species belonging to these genera have already proved their ability to produce volatile components such as TCA in cork and cork stoppers in other studies around the world. Other species, however, have only appeared in unboiled cork, such as Trichoderma sp, Fusarium sp, Gelasinospora brevispora, Sordaria humana, and two basidiomycetes indicative of high humidity and poor cork storage conditions, Coprinus xanthothrix and Schizopphyllum commune. Conclusion: The natural cork stoppers industry requires the use of healthy cork boards of a good mycological quality to avoid denaturing corked food products.
Halophilic and halotolerant microorganisms: bio-factories of amazing compounds with an outstanding biotechnological potential
Introduction: Halophilic and halotolerant microorganisms are a group of extremophiles that are adapted to survive in high salt conditions. The successful occupancy of life form in such unfavorable environment has attracted and focused the attention of the scientific community for potential applications of their bioactive molecules. This mini-review summarizes some potential applications of those microorganisms in biotechnology that have been highlighted in specialty journals with a significant impact factor. Methods: To carry out this exploratory review involve the online survey including and coordinating information from convincing publications on in vitro works by means of electronic database and scientific tools such as Google Scholar, PubMed, Springer link and Science Direct. Results: This systematic review was structured based on the years of the publication of the journals, the categories and the used keywords. The results showed the potential of production of highly stable biomolecules by halophilic and halotolerant such as extreme-enzymes, antimicrobial compounds, antioxidant, biomolecules with anticancer and anti-diabetic activities, compatible solutes, carotenoid pigments, exo-polysaccharides, and biosurfactants products which can be used in medicine, agriculture, bioenergy and other industries and that can resolve current challenges. Conclusion: Halophiles and halotolerant microorganisms have the potential to offer significant opportunities for biotechnology. Their high metabolic diversity and highly stable biomolecules suggests that they will prove even more valuable in the future.
Occurrence of fungal species associated with grapevine dieback in western of Algeria (case of the Tlemcen region)
Introduction: The present study carried out in the Tlemcen region (In four different farms) between 2021 and 2022, focuses on the isolation, identification and characterization of phytopathogenic fungi responsible for fungal diseases of the vine (Vitis vinifera L.). Methods: Out of a total of 60 samples, the isolation of fungi from the aerial part and underground of symptomatic trees revealed the presence of significant fungal biodiversity on the culture medium. Results: Sixteen fungal species of different genera have been isolated and identified, namely: Aspergilus fumigatus, Aspergilus niger, Alternaria alternata, Penicillium sp., Penicillium communeon, Penicillium solitum, Fusarium sp., Fusarium solani, Fusarium culmorum, Pythium sp., Pythium ramorum, Diplodia corticola, Botrytis cinerea, Mucor racemosus, Rhiropus stolonifer and Monilia fructicola. The two species Diplodia corticola and Botrytis cinerea are strongly present in the vascular tissues of the collar and trunk, with 9 and 12%, respectively. Conclusion: As well as species of the Fusarium genus with 18% in the rhizosphere of symptomatic trees.
Anti gastric ulcer activity of the root bark of Osyris Alba L skell Santalaceae from the south ouest of Tlemcen (Algeria)
Introduction: The work presented in this paper contributes to the valuation of the wound healing power of the decocted of the plant Osyris alba L Skeel which is belonging to the Santalaceae family, its root is traditionally used by the local population for treatment of gastric ulcers, rheumatism and its decoction is taken as a fortifier. Methods: The qualitative analysis of phenols compounds was carried out by high performance liquid chromatography coupled with a diode array detector on a reversed phase column (RP-HPLC-PDA). The gastric antiulcer activity consisted of causing gastric ulcers by administering orally the ulcerogenic mixture for each Wistar rat and then to evaluate the healing power of Osyris alba decoction in the treatment of gastric ulcers. Results: According to the study the curative effect of the aqueous extract on gastric ulcer, evaluated in Wistar rats, showed a wound healing power with an estimated percentage inhibition of 98.67% which exceeds the ranitidine (standard). Conclusion: These results have enabled us to promote Osyris alba L Skeel as an antiulcer plant which is due to the presence of phenolic compounds (Gallic acid and Quercetin) which are analyzed by HPLC DA.
α-amylase inhibitory effect of several varieties of cheese: Gouda, Gruyere, Edam, Emmental
Introduction : Cheese is a highly nutritious fermented milk product with diverse flavor and texture. As a functional food it has gained a lot of attention in recent times because of their health benefitting properties. Methods: In the present study, four varieties of cheese (Gouda, Gruyere, Edam and Emmental) are tested for their antidiabetic effect. In fact, an aqueous extract is prepared by maceration of cheese pieces in phosphate buffer. Inhibitory effect against α-amylase of extract, pH and proteins content are investigated. Results : Results show pH value between 5.2 and 5.9 with protein content between 4.5 and 9.14%. Inhibitory effect against α-amylase is moderate with IC50 values of 49.29±4.02, 38.5±6.05, 70.66±12.52 and 25.9±6.82 mg.mL-1 for Goud, Gruyere, Edam and Emmental respectively. Conclusion : In fact, we can conclude that Emmental cheese has the best inhibitory effect on porcine pancreatic α-amylase compared to Gouda, Gruyere and Edam cheeses.. Investigation of active ingredient in cheese especially in Emmental may be interesting.