Journal of Natural Product Research and Applications
JNPRA publishes high-quality manuscripts of original works in all aspects of natural compounds of plants, algae, microbes, and animals. The journal is peer-reviewed and publishes two issues per year. Authors are encouraged to submit complete, unpublished, original works that are not under review in any other journals. All submitted manuscripts are subject to initial examination by the editors for the first step and peer-reviewed by at least two anonymous expert referees for the second step. Major topics of JNPRA enclosed are: Biology and Biological Chemistry: Biochemistry, Biology, Microbiology, Cell and Molecular Biology, Biotechnology, Neuroscience, Phytochemistry. Medicinal Chemistry: Toxicology, Pharmacology, Pharmacognosy, Nutrition and Health, Therapeutics, Bioactive Compounds, Detoxification, Biological activities. Pharmaceutical Formulations: Formulations from natural products, Analysis, Quality Controls. Agricultural and Food Chemistry: Food Technology, Agricultural and Food Safety, Food analysis. Applications of Natural Resources: Cosmetics, Dietary Supplements, Nutraceuticals, Biopharmaceutical Science, Waste and co-product valuation. Toxicology: Detoxification, Toxicological medicine, Risk assessment, Safety evaluation, environmental health, Pollution and public health, Waste and co-product valuation. Traditional Medicine and Ethnopharmacology: folkloric medicine, ethnobotany, ethnopharmacology. All papers that treated the biological activities of crude extracts are accepted after structure elucidation of active molecules. Papers related to In Silico Design of Bioactive Molecules are welcomed for publication. The paper reporting new compounds and manuscripts describing their biological activity are more solicited for publishing in our journal. Frequency: The Journal of Natural Product Research and Applications publishes a twice per year (April and November). ISSN (online): 2773-4188 DOI: https:// doi.org/10.46325/JNPRA Submission: all peer review is single-blind and submissions should be made by sending over our website (click to submit). Template JNPRA: click here to download Journal charges: there are no publication fees to publish in the Journal of Natural Product Research and applications (JNPRA). Format: Currently, the Journal of Natural Product Research and Applications is publishing online-only format. Manuscript categories: the Journal of Natural Product Research and Applications publishes full Papers, short communications, reviews, letters to the editor, notes, and book reviews.
University of Abou Bekr Belkaid at Tlemcen
Bioactive Compounds and Medicinal Applications of Moringa oleifera: A Review
Moringa oleifera is increasingly recognized as a "miracle tree" due to its exceptional nutritional and medicinal properties. This plant serves as a valuable natural resource, not only enhancing dietary intake but also aiding in the prevention and treatment of a variety of health conditions. Rich in easily absorbable proteins, iron, calcium, potassium, and essential vitamins such as A, C, and E, M. oleifera also contains significant levels of bioactive compounds, including polyphenols. Various parts of the plant such as seeds, roots, leaves, flowers, bark, and buds, demonstrate a wide array of pharmacological activities, including anti-inflammatory, antimicrobial, anticancer, antihypertensive, antihyperlipidemic, antidiabetic, hepatoprotective, and neuroprotective effects. This review aims to provide a thorough overview of the current global research on M. oleifera, covering its phytochemical composition, pharmacological properties, toxicological data, and ethnomedicinal uses. Furthermore, it emphasizes its potential phytopharmaceutical applications and presents a case study illustrating its effectiveness as a natural coagulant in water purification. By synthesizing these findings, this review aims to inspire further research into the nutritional and therapeutic benefits of M. oleifera.
Moringa oleifera, Nutritional properties, Therapeutic applications, Bioactive compounds, Medicinal plants.
Ibtissem BENGHALEM¹, Zoubida MAMI-SOUALEM¹, Radjaa Kaouthar MEZIANE¹, Mansouria Souria BENDEDDOUCHE².
Copyright (c) 2026 https://creativecommons.org/licenses/by-nc/4.0
Phytochemical and antioxidant activity of male and female buds and pollen grains of Pistacia atlantica
To enhance the different parts of Pistacia atlantica, male and female buds, and pollen grains were selected to quantify the levels of phenolic compounds and evaluate their antioxidant properties. The results showed that the best yield (30.60 ± 2.82%) was in male buds. Noting that the crude extracts of male and female buds were rich in total phenols (636.83 ± 4.72 and 648.97 ± 4.80 mg GAE/g DM) and condensed tannins (4.10 ± 0.53 and 6.02 ± 0.62 mg CE/g DM), respectively, while the crude extract of pollen grains was rich in flavonoids (15.53 ± 1.20 mg CE/g DM). The study of antioxidant power revealed that the crude extract of male buds has the best total antioxidant capacity (TAC) (115.08 ± 3.38 mg AAE/g DM), and the best effects of reducing power (EC50 = 0.094 ± 0.003 mg/mL) and β-carotene bleaching (EC50 = 0.533 ± 0.14 mg/mL). HPLC-DAD analyses revealed the presence of rutin in pollen grains and male bud extracts and catechin in female bud extract. These findings highlight the male buds of P. atlantica as the most promising part of the plant, suggesting their valuable use in phytopharmaceutical and nutraceutical applications.
Pistacia atlantica, Buds, Pollen grains, Phytochemical study, Antioxidant activity.
Asmaâ Dich, Waffa Abdelmoumene, Larbi Belyagoubi, Assia Benmahieddine, Imad Abdelhamid El Haci, Ikram Belhocine, Imane Nabib, Nabila Belyagoubi‑Benhammou
Copyright (c) 2025 https://creativecommons.org/licenses/by-nc/4.0
Therapeutic Efficacy, Regulatory Frameworks, and Socioeconomic Implications of Plant-Based Anti-Inflammatory Treatments: A Critical Review
This critical review evaluates the evolving landscape of botanical anti-inflammatory therapeutics by synthesizing global literature published between 2014 and 2025. Encompassing data from Africa, Asia, and the Americas, the analysis elucidates the interplay between pharmacological efficacy, regulatory frameworks, and socio-economic drivers in plant-based therapeutics. Key findings indicate that specific bioactive constituents, notably alkaloids and flavonoids, exhibit substantial promise in mitigating inflammation associated with chronic pathologies such as rheumatoid arthritis and inflammatory bowel disease. However, a significant translational gap remains; while preclinical data is robust, there is a paucity of large-scale, randomized human clinical trials necessary to validate these findings for mainstream medical use. Furthermore, the global integration of these therapies is impeded by fragmented regulatory standards and persistent ethical concerns regarding biopiracy and equitable benefit-sharing, despite the critical role these affordable remedies play in underserved communities. The review concludes that the future of integrative healthcare depends on harmonizing global policies and integrating traditional practices and modern science through the adoption of rigorous clinical validation and advanced technologies, including artificial intelligence and nanomedicine.
Plant-based treatments, inflammation, ethnopharmacology, regulatory frameworks, socio-economic impact, integrative medicine.
Meknassia DEBBAL, Ismahane BOUCHNAFA, Imane GHELAI
Moussa BELHADJ, Houssam BOULENOUAR , Tarik Mohammed CHAOUCHE (2)
Date Seeds in Cosmetics: A Synthesis of Antioxidant, Photoprotective, and Regenerative Effects
The contemporary cosmeceutical sector confronts significant challenges in aligning environmental responsibility with therapeutic effectiveness, catalyzing the emergence of "Circular Cosmeceuticals"—bioactive skincare components sourced from discarded agricultural materials. Substantial knowledge deficits persist regarding which specific by-products can simultaneously deliver ecological advantages and clinically validated skin benefits. This narrative review investigates Date Seed Oil as an exemplary ingredient within sustainable cosmeceutical development, with particular emphasis on its photoprotective and regenerative capacities. A methodical literature search was performed utilizing PubMed, Scopus, and Web of Science databases, targeting peer-reviewed publications released between 2018 and 2025. Selection criteria prioritized investigations examining Date Seed Oil's biochemical composition, cellular mechanisms, and dermatological applications. Evidence demonstrates that Date Seed Oil operates as a cellular antioxidant by activating Nrf2-mediated defense pathways, thereby counteracting UV-triggered oxidative damage beyond simple radiation absorption. Its synergistic enhancement of sun protection factor occurs through stabilization of chemical UV filters against photodegradation. Concerning skin barrier restoration, the oil promotes endogenous lipid production critical for stratum corneum integrity. Analytical assessment identifies oxidation susceptibility as the principal constraint while emphasizing nanoencapsulation technologies, especially phospholipid vesicle systems, as fundamental solutions for maintaining ingredient potency. This evaluation positions Date Seed Oil as a multifunctional cosmeceutical agent that successfully merges agricultural waste transformation with scientifically substantiated dermatological intervention.
Circular Cosmeceuticals; Date Seed Oil; Photoprotection; Nrf2 Signaling; Nanotechnology
Souad SENHADJI , Ismahane BOUCHNAFA , Imane GUELAI , Farah HADDOUCHI, Tarik Mohammed CHAOUCHE
In silico study of the biological properties of four sterols identified in Anabasis articulata plant
The search for novel therapeutics with diverse pharmacological activities has led to increased interest in natural products. In this investigation, we aim to explore the potential medicinal properties of four sterol-type molecules found in the medicinal plant Anabasis articulata (Forssk) using in silico methods. The molecules of interest are cholestanol, β-sitosterol, stigmasterol, and 26-hydroxycholesterol. To assess their therapeutic potential, we employ several theoretical methods. Firstly, molecular docking simulations are utilized to model the interactions between these molecules and target proteins associated with antioxidant, anti-inflammatory, anti-cancer, and neurodegenerative effects. The selected targets include Xanthine Oxidase (XO) for antioxidant activity, Cyclooxygenase-2 (COX-2) for anti-inflammatory effects, Estrogen Receptor Alpha (ERα) for anti-cancer properties, and Acetylcholinesterase (AChE) for neurodegenerative disorders. Visual analysis of these docking results aid in understanding the binding modes and potential mechanisms of action. Furthermore, we predict ADMET pharmacokinetic parameters and physicochemical properties to evaluate the drug-likeness of the compounds. Our findings reveal promising interactions between the sterol-type molecules and target proteins associated with various therapeutic effects. Furthermore, through rigorous evaluation of drug-likeness rules, we identify compounds with favorable structural and physicochemical characteristics for potential drug development. This comprehensive computational approach offers valuable insights into the medicinal properties of Anabasis articulataderived sterol-type molecules, paving the way for further experimental validation and pharmaceutical exploration.
Anabasis articulata, Sterols, Biological activities, Molecular docking simulation, ADMET.
Ikram Lakehali, Fatiha Ould Ali, Nabila Belyagoubi-Benhammou
Copyright (c) 2024 https://creativecommons.org/licenses/by-nc/4.0
Insomnia and therapeutic alternatives: Survey on the use of phytotherapy by the northern Algerian population
The number of people suffering from sleep disorders is constantly increasing over the years. To preserve their health and avoid the use of conventional drugs (addiction, undesirable effects, toxicity, etc.), phytotherapy remains a good alternative. This study aims to identify the medicinal plants used by the Algerian population to treat sleep disorders. This was an ethnobotanical survey carried out using an electronic questionnaire concerning the population and their knowledge of the medicinal plants used, followed by bibliographical research on the plants mentioned. 197 people from the north of Algeria took part in this study, which revealed the use of 33 medicinal plants and 7 herbal products to regulate sleep or improve its quality. The use of medicinal plants is widespread in Algeria, prompting researchers to carry out various phytochemical and pharmacological studies to ensure their efficacy and detect any interactions (plants/drugs), adverse effects or toxicity.
Insomnia; Medicinal plants; Sleep disorders; Phytotherapy
Investigation of the preliminary compounds; Assessing the antioxidant and in-vitro antibacterial activities of Inula confertiflora leaf extracts
Medicinal plants, from those traditionally utilized by healers in underdeveloped nations to those employed in the manufacturing of contemporary synthetic medications worldwide, are essential in the treatment of a wide range of illnesses. Ethiopians have long utilized the leaves of Inula confertiflora plants to treat diseases pertaining to the skin and eyes. This encouraged us to look at the yield of crudes extracted using various polar index solvents, look at the existence of precursor compounds in the plant's leaf sections, and evaluate the ability of preliminary compounds to scavenge free radicals from DPPH and their antibacterial properties against gram-positive and gram-negative bacterial strains. Following the extraction processes of the plant soaking, filtering, and concentrating the crudes, the highest yield of crude was produced by ethanol in moderately polar solvents (3.64 mg) water with a higher polarity index (5.74 mg) and acetone in lower polar solvents (6.28 mg), which produced a relatively lower yield. The UV-Vis spectroscopic results of the ethanol extracts showed they contained a wide range of preliminary bioactive functional groups, like phenols and flavonoids, and their derivative compounds. Additionally, the ethanol extracts' inhibition zone was marginally higher than that of the water and acetone extracts', indicating that the preliminary compounds extracted in a higher polar solvent—water—and a moderate polar solvent—acetone—were obtained in the ethanol extracts. In conclusion, the presence of higher total content of the phenolic and flavonoids, higher radical scavenging efficiency in DPPH solution, and greater zone of inhibition in-vitro antibacterial activities displayed by the preliminary compounds extracted by ethanol and water extracted from the leaf of I. confertiflora corroborate the traditional uses by the local people against various diseases.
Medicinal plants; secondary metabolites; Inula confertiflora; antioxidant and antibacterial activity.
Effect of Citrullus colocynthis oil on the lipid profile of obese male rats
Citrullus colocynthis is a plant of the cucurbitaceae family native to arid soils. It contains around 17℅ of oil, rich in essential polyunsaturated fatty acids with regulatory powers for certain metabolic pathologies such as hypercholesterolemia. The aim of this work is to test the effect of colocynth oil (CO) on hepatic lipids in Wistar rats fed hyper-fatty diets supplemented with (CO), compared with other rats fed control diets based on 8 ℅ sunflower oil (SO). To this end, male Wistar rats weighing 80±5g were divided into four batches. The rats were fed different diets (R) for 2 months (D1: 16℅ casein + 8℅ SO, D2: 16℅ casein+ 8℅ CO, D3: 16℅ casein+ 30℅ fat+ 8℅ SO, D4: 16℅ casein+ 30℅ fat+ 8℅ CO), the weights of the rats and the quantity of food ingested were taken daily, at the end of the diet the rats were dissected and the livers recovered for hepatic lipid assays using the folch method. The results showed a reduction in triglycerides in the liver of rats fed diets containing oil of colocynth (37%) and reduced blood triglyceride (12%) and cholesterol (6%), whereas rats fed high-fat diets had elevated hepatic and blood lipid levels. Colocynth oil has a corrective and/or protective effect on obesity and its complications by preventing the accumulation of fat in the liver, thereby preventing hepatic steatosis.
Obesity; hepatic steatosis: colocynth oil; liver; PUFAs.
Sidi Mohammed YAZIT, Fouzia AMAMOU, Radjaa Kawtar MEZIANE, Zoubida SOUALEM-MAMI, Hamadi Abderrahmane LAZZOUNI, Daoudi CHABANE SARI
Insecticidal activity of diatomaceous earth on larvae and adults of Ceratitis capitata Wied., (Diptera; Tephritidae), citrus pests against ecological control
To assess the toxicity effect of diatomaceous earth on larvae and adults of Ceratitis capitata, we subjected them to different doses to control their mortalities. Adults of Ceratitis capitata were exposed to diatomaceous earth at different concentrations: 0mg/cm2; 2,6mg ; 2,8mg ; 3mg ; 3,2mg ; 3,4mg ; 3,6mg ; 3,8mg and 4 mg/cm2. We monitored their mortality rate every 12 hours up to 96 hours of exposure. The larvae were also exposed to different concentrations of the diatomaceous earth mixed with sawdust: 0, 100, 120, 140, 160, 180, 200, 220 and 240 mg/cm3. We monitored their mortality rate every 12 hours up to 96 hours of exposure before pupation. The insecticidal activity of diatomaceous earth on Ceratitis capitata has offered us highly significant results on the different stages (larvae and adults). Indeed, we obtained CL50 of 158.29 mg/cm3 for larvae and 3.262mg/cm2 for adults. The mortality average of Ceratitis larvae increased with the doses of diatomite used, reaching its maximum at the dose of 240mg/cm3. The mortality average of Ceratitis adults after exposure to diatomaceous earth showed an ascending curve which started from the dose of 2.60mg/cm2 with a mortality average of 43.3% through repetition, reaching its maximum of 10 dead individuals through the repetition of the dose of 4mg/cm2. These results allow us to propose this natural product as an alternative to insecticides hitherto used to fight Ceratitis capitata, which will better protect our health and environment.
Diatomaceous earth, Ceratitis capitata, mortality, toxicity, control.
Yassine Bounouira*, Abdellah Mohamed Cherif
Siham Tchouar , Nassira Gaouar Benyelles
Assessment of the Soil Bulk Density in the Morneg Region (Tunisia)
Soil compaction constitutes a serious problem for the development and production of crops. This settlement is generally caused by the multiple passages of machinery, particularly in excessive moisture conditions. In this context, a study of the soil bulk density variation as a function of the number of passages, moisture and the state of the soil (tilled or not) are studied. The density study is conducted on a plot located in Morneg-Ben Arous (Tunisa). Experimental tests applied to a sandy soil showed that the density increased as a function of the number of tractor passes and the depth soil, under different moisture levels. In fact, two passes, at a depth 20 cm, under moisture conditions varying between 3-7% and 12-16%, can increase the soil density by 12% and 37% respectively compared to the density of a tilled soil. In addition, following 3 passes, a compaction (Bulk Density =1.6g/cm3) of the wet soil (moisture 12-16%) at 40 cm depth was detected.
Bulk Density, Moisture, Compaction, Agricultural, Sandy soil.
Phycochemistry study and antimicrobial activity of Spirogyra freshwater green microalgae from Algeria
In Algeria, algae have not been adequately explored for their potential as a source of bioactive substances. In this context the blooms of green alga Spirogyra sp. were collected from freshwater habitats in Oued El Mellah near M’sila province of Algeria. Hexane, acetone and ethanol crude extracts were screened for antimicrobial activity against Staphylococcus aureus, Listeria monocytogenes, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Candida albicans. The contents of total soluble sugars, soluble proteins, total lipids and phenolic acids were also determined. The present findings revealed that the highest extraction yield was obtained from ethanol (4.93%) compared to acetone (3.67%) and hexane (2.33%). The contents of phycochemical compounds were 32.5% of soluble carbohydrates, 15.84% of lipids, 15.6% of phenolic acids and 5.16% of proteins. All extracts exhibited better and stronger antibacterial activities against the five pathogenic bacteria with inhibition zones diameter ranged from 13.5-29 mm for acetone extract, from 13-25 mm for ethanol extract and from 12.5-20.5 mm for hexane extract at 7.5 µL. The minimal inhibitory concentration (MIC) was in the range of 15-40 mg mL-1. The acetone extract was more effective than others extracts with MIC value of 15 mg mL-1 for all bacteria. These findings indicate the presence of promising antibacterial compounds originating from microalgae, which can be exploited for the production of new antimicrobial agents.
Oued El Mellah; algae; Spirogyra; phycochemical componds; antimicrobial activity
Fatima Zohra AISSAOUI, Zahrat El Oula BENAMAR
In vitro antioxidant and antidiabetic activities of the hydro-acetone extracts of Ricinus communis and Teucrium polium from Tlemcen area
The aim of the present study was to determine total phenolic and total flavonoid contents in hydro-acetone extracts from the roots of Ricinus communis and the aerial part of Teucrium polium, and to determine in vitro their antioxidant and antidiabetic activities using DPPH, FRAP, and α-amylase activity assay, respectively. The obtained results reveled that R. communis roots hydro-acetone extract contains high contents of polyphenols (499.55 ± 0.02 µg GAE/mg DE) and flavonoids (28.21 ± 0.01 µg Cat eq/mg DE) than T. polium aerial part extracts. Both plant extracts showed in vitro antioxidant and antidiabetic activities, where R. communis showed a potent free radical scavenging activity (DPPH IC50=2.01 ± 0.01 µg/mL) and high iron reduction capacity (FRAP EC50=41.32 ± 0.01 µg/mL) more than T. polium extract (DPPH IC50= 7.40 ± 0.01 µg/mL; FRAP EC50=74.63 ± 0.01 µg/mL). In addition, in α-amylase activity assay T. polium was more efficient in inhibiting this enzyme (EC50= 924 ± 0.19 µg/mL) compared to R. communis extract (EC50= 1300 ± 0.03 µg/mL).
Ricinus communis; Teucrium polium; total polyphenol contents; DPPH; FRAP; α-amylase.
Nabila BENARIBA, Zohra FEKHIKHER, Radia BRIXI-GORMAT, Hanane BENRAMDANE, Houda YOUBI , Ibtissam KOUAR , Hanane MILOUDI , Soumia MEHARRAR
Maceration, microwave assisted and ultrasound assisted extraction of phenolic compounds from Tunisian Limoniastrum monopetalum: kinetic modeling and chemical analysis
Maceration, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) were investigated in this study, focusing on the selectivity towards phenolic compounds in ethanol extracts from Limoniastrum monopetalum leaves using the Folin-Ciocalteu method and kinetic models of extractions. Moreover, several phenolic compounds were identified by RP-HPLC. Results showed that total polyphenol compounds (TPC) under the MAE conditions (ethanol, 700 W microwave power, 60 s extraction time, and 1 mL/g solvent-to-solid ratio) were 75.707 mg GAE/100 g dried sample in only one minute of extraction. We conclude that MAE is a promising extraction method for polyphenols. Model results were correlated with mathematical models of the extraction process. Six phenolic acids and three flavonoids were identified by HPLC–DAD. MAE is a valuable and green analytical methodology for the investigation of phenolic components in natural plants.
Limoniastrum monopetalum; RP-HPLC; phenolic compounds; UAE; MAE.
Phytochemical study and evaluation of α-Amylase inhibitory effect of extracts from Cistanche violacea Desf.
Cistanche violacea Desf. is a species of the family Orobanchaceae. It is an angiosperm dicotyledonous holoparasitic plant, which lacks chlorophyll. This work focuses on the search for an inhibitory effect of the enzymatic activity of α-amylase, in the presence of the crude water-methanol extract, of the underground part of C. violacea, prepared by maceration or decoction and its ethyl acetate fractions. Qualitative phytochemical tests and quantitative assays of phenolic compounds of the prepared extracts were performed. The effect of each extract on the activity of the α-amylase enzyme was evaluated under optimal conditions (pH 6.9 and T° 37°C). The results of phytochemical tests revealed the presence of tannins, flavonoids, free quinones, terpenoids and reducing compounds. The determination of phenolic compounds showed that the ethyl acetate extract prepared by maceration was the richest in total polyphenols and flavonoids with contents of the order of 82.937±6.750μg EqGA/mg D.W and 112.345±25.719μg EqC/mg D.W respectively. While the crude hydromethanolic extract prepared by maceration presented the highest content of condensed tannins (525.5±77.5μg EqC/mg D.W). On the other hand, the ethyl acetate extract prepared by decoction of the underground part of C. violacea showed a better inhibitory activity of the α-amylase enzyme, with IC50 value of 1.88 mg/ml, compared to the other extracts tested. This activity remains low compared to acarbose (IC50 = 0.132 mg/ml). We concluded that the roots of C. violacea exerts an inhibitory effect on α-amylase activity and thus on the regulation of hyperglycemia through a decrease in intestinal glucose absorption
Diabetes mellitus, Cistanche violacea, α-amylase, phenolic compounds, inhibitory effect.
Antioxidant and cytotoxic activities of extracts from Haloxylon scoparium Pomel
Haloxylon scoparium Pomel is a plant known to possess anti-cancer properties as well as anti-plasmodial and larvicidal activities. The aerial parts are used in traditional medicine to treat disorders and problems of the eye and vision, digestion problems, dermatoses, scorpion pitting. The antioxidant and anticancer activities of different extracts (dichloromethane, ethyl acetate and butanol) from flowers and stems were studied. The flowers exhibited strong antioxidant activity using the oxygen radical absorbance capacity method and a cell-based assay. The most interesting results are obtained with the ethyl acetate extracts of the flowers active with an ORAC value of 6.6 μmol TE / mg, followed by the butanol extract of the same organ (ORAC value of 6.22 μmol TE/ mg). The experiments carried out using the cellular antioxidant protocol reveal a very strong antioxidant activity, particularly for the ethyl acetate and butanol extract of the flowers with an IC50 value of 0.029 ± 0.004 µg/ mL and 0.3 ± 0.1 µg/ mL respectively . The cytotoxic activity (Resazurin tests) was found to be moderate against A-549 (lung carcinoma) and DLD-1(colon carcinoma) cells, with IC50 values of 110 ± 13 and 117 ± 14 μg/ mL, respectively. All the results obtained constitute a scientific justification for the use traditional of the plants studied and once again confirms the relevance of the remedies traditional in the treatment of many diseases.
Antioxidant activity; Cytotoxic activity; Haloxylon scoparium Pomel.
Colorectal cancer and natural products: Role of Marrubium vulgare L. and Zingiber officinale Roscoe
Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths, it involves mainly the colon and rectum. It is one of the most common malignancies in adult men and women. The highest incidence is found essentially in economically developed countries. In Algeria, CRC is the second most diagnosed cancer, this multifactorial disease is associated with several risk factors such as advanced age, diabetes, genetic susceptibilities, unhealthy diet, obesity, and smoking. Some herbal medicines have been widely used with or without chemotherapy to treat CRC; these natural products demonstrated some efficacy in improving patients’ survival and quality of life. In this review, we introduce the most common risk factors associated to CRC etiology, then, we highlight the anti-CRC activity of two plants used in traditional medicine in Algeria, which are Marrubium vulgare L. and Zingiber officinale Roscoe., and discuss potential anti-cancer mechanisms of these plants.
Colorectal cancer, risk factor, secondary metabolites, Marrubium vulgare L., Zingiber officinale Roscoe.
Copyright (c) 2023 https://creativecommons.org/licenses/by-nc/4.0
Lentisk fruits (Pistacia lentiscus L.) as sources of phytochemicals with potential health benefits: A review
The field of phytochemistry is increasingly interested in the use of plants in order to discover new therapeutic treatments, by scientifically proving their role, as popular remedies against diseases. Pistacia lentiscus L. is a medicinal and aromatic plant belonging to the Anacardiaceae family. The distribution of this plant includes Mediterranean area. Indeed, it is very used in Algerian traditional medicine. Different parts of this specie have been used in traditional medicine to treat some diseases, such as respiratory problems, disorders of the digestive system, hypertension, coughs, sore throats, eczema, stomachaches, kidney stones and jaundice in many countries. Scientific findings also revealed the wide pharmacological activities from various parts of this specie, such as antioxidant, anti-inﬂammatory, antimicrobial, antiviral, antimutagenic, antidiabetic, hepatoprotective and anticancer activities. Many phytochemical constituents like phenolic compounds, terpenoids, fatty acids, and sterols have also been extracted and identified from diﬀerent parts of Pistacia species in many recent studies. The objective of this review was to screen the biological properties of P. lentiscus extracts and oils of fruits. Starting from this, we had first researched the phytochemistry composition of this part collected from different geographical areas and then reported the traditional uses. In the second part, we had presented the scientific discovery of its potential and biological activities.
Pistacia lentiscus. L; Traditional medicine; Phytochemistry; Biological activities.
Evaluation of oxidant and antioxidant status in obese children of Tlemcen (Algeria)
Oxidative stress was evaluated in obese children in Tlemcen, Algeria without co-morbidities. The study selected a 40 obese children and 40 non-obese children aged 6 to 12 years. The rate of markers of oxidative damage (malondialdehyde [MDA], and plasma hydroperoxide [Hp]) and evaluation of antioxidant defense, such as the oxygen radical absorbance capacity [ORAC] and Catalase were determined. Children were categorized by the body mass index (BMI). The levels of plasmatic MDA and Hp were higher in obese children compared with non-obese children. In contrast and as expected, the values of CAT and the ORAC was decreased in obese subjects compared with control subjects. Obesity affecting children severely is related to oxidative stress. Thus, a diet rich in foods with high antioxidant capacity in addition to a low-calorie diet is essential for the treatment of obese children.
Antioxidants; body mass index; children; obesity; oxidative stress.
Extraction characterization and investigation of breadfruit seed oil for possible pharmaceutical application
This work aimed at extracting, characterizing and evaluating breadfruit seed oil for potential pharmaceutical applications. The breadfruit oil was purified by degumming, bleaching, neutralization and enzyme hydrolysis. The oil was characterized for the yield, pH value, iodine, acid, peroxide and saponification values and fatty acid composition. Results obtained were interpreted with respect to their relevance in pharmaceutical formulations.The percentage yields of the oil from the soxhlet procedure was 23.54 % while the yield from the cold maceration extraction method was 13.69 %. The iodine, acid, peroxide and saponification values were, 17.50 ± 0.05, 3.40 ± 0.33, 7.20 ± 0.08, and 236.46 ± 0.02 respectively. Gas chromatographic- infrared studies identified five polyunsaturated long chain fatty acids. The breadfruit oil has good edible properties by virtue of its moderate acid and peroxide values. Its high iodine and low saponification values may necessitate the addition of antioxidants in pharmaceutical formulations containing breadfruit oil. The results obtained in this work suggest that breadfruit oil has good potential for pharmaceutical applications.
extraction; polyunsaturated; chromatography; degumming; hydrolysis; pharmaceutical.
Insights on essential oils opportunities in the therapy of multidrug resistant Fungi
Essential oils: definitions and chemical complexity Essential oils (EOs), sometimes referred to as volatile oils, essences, etheric oils, or aetheroleum, are derived from various parts of plants, including flowers, leaves, stems, roots, and seeds. They contain highly concentrated volatile compounds and are utilized for a range of purposes such as aromatherapy, massage, skincare, and as natural remedies for different health problems. The composition of EOs can vary based on the plant species and the part of the plant from which it is extracted. Some popular types of essential oils include lavender, peppermint, eucalyptus, lemon, and tea tree oil. (Gentile et al., 2020; Zuzarte and Salgueir 2015). According to the AFNOR NT 75-006 standard (February 2006): "An essential oil is a product obtained from plant row-material, either by steam distillation or by mechanical processes from epicarp of lemons, either by dry distillation, and which is separated from the aqueous phase by physical processes. The most frequent method for obtaining essential oils is through distillation, however, other techniques such as enfleurage (using fat for extraction), maceration, solvent extraction, and mechanical pressing are employed for specific products. EOs complexity includes thousands of biomolecules as secondary metabolites with a major role of attracting pollinators, deterring predators and protecting plants from harmful pathogens. Essential oils are primarily made up of terpenes produced through the mevalonate pathway, including monoterpenes (monoterpene hydrocarbons and oxygenated monoterpenes), and sesquiterpenes (sesquiterpene hydrocarbons and oxygenated sesquiterpenes). Additionally, they contain phenolic compounds that are derived from the shikimate pathway. Due to their chemical composition, essential oils possess a range of biological activities, including antioxidant, anti-inflammatory, antimicrobial, antifungal, among others, which make them of significant interest in the food and cosmetic industries and for human health applications. Multidrug resistant Fungi and Essential oils Multidrug-resistant fungi (MDR fungi) are fungal pathogens that have developed tolerance to a range of drugs. The recent emergence of these pathogens resistant to multiple classes of antifungal drug is a serious concern. Ergosterol is a type of lipid that plays a crucial role in maintaining the fluidity and permeability of fungal cell membranes, as well as ensuring the proper function of integral membrane proteins. It is also necessary for the survival of fungal cells. (Leber et al., 2003; Tatsumi et al., 2013; Song et al., 2016; Scorzoni et al. 2017). Ergosterol is a potential target for the development of antifungal agents against multi resistant fungi (e.g: Candida spp.; Aspergillus fumigatus) either by inhibiting its biosynthesis or by binding to it, causing formation of pores in the membrane (Scorzoni et al. 2017). Mechanisms of resistance to clinically relevant classes of antifungal drugs include the Ergosterol sequestration (Polyene’s drug class), the Inhibition of ergosterol biosynthesis (Azole’s drug class) and Inhibition of β-1,3-glucan synthase (Echinocandins’s drug class) (Berman and Krysan., 2020). It is important to note that multidrug resistance fungal strains is a growing public health concern and new treatments are needed to combat these infections. Some of the most common multidrug-resistant fungal species include, among others: Candida spp., Aspergillus fumigatus, Cryptococcus neoformans, (Fernandes et al., 2022; Marquez and Quave., 2020). Antifungal activity of essential oils against multi resistant antifungal pathogens is well documented (Nazzaro et al., 2017; Singh and Pulikkal., 2022; Yadav et al., 2022). The toxic effect of essential oils and their components regarding fungal cells is attributed to their capability causing harmful effects to the cell walls and membranes, triggering the damage to cellular organelles, the coagulation of cytoplasm and leakage of macromolecules (Burt 2004; Hyldgaard et al. 2012). The oils' ability to dissolve in fats as lipophilic components, allows them to penetrate the cell wall and harm the layers composed of polysaccharides, fatty acids, and phospholipids, making the cells more permeable (Helal et al. 2006; Rammanee and Hongpattarakere 2011; Dwivedy et al. 2016). In our opinion, investigations on the effect of essential oils against multi resistant fungi should be conducted in an intense way. Moreover, a multidisciplinary approach including the effect of selected essential oils, pure phytochemicals isolated from essential oils, the combination between different essential oils and/or pure phytochemicals with the known used antifungal drugs as well as the correlation between the inter-reactions of those oils and/or phytochemicals with fungal biological molecules in the fungal cell wall and cell membrane (Ergosterol, Chitin....) could understand in the depth and in detailed manner the mode of action and therefore valorise adequately their effect on multi resistant fungi.
1er Séminaire National sur les Plantes, Molécules Bioactives et Valorisation (SN-PMBV-2022)
Le 1er Séminaire National sur les Plantes, Molécules Bioactives et Valorisation (SN-PMBV-2022), se tiendra cette année les 22 et 23 Novembre 2022, à l’Ecole Normale Supérieure d'Enseignement Technologique (ENSET) de Skikda. Ce séminaire sera pour nous l’occasion d’accueillir des scientifiques algériens, travaillant dans différents domaines; les thématiques concernent notamment la valorisation biomoléculaire et les produits naturels, l’extraction, la séparation et la caractérisation des molécules bioactives, les activités biologiques et leurs applications en biotechnologie et en fin la chimie verte. Ce webinaire verra cette année la participation de soixante-dix-neuf participant(e)s dont; quatre conférences plénières, quarante communications orales et trente-cinq communications par affiches. Les membres du comité scientifique et d’organisation tiennent à remercier tous les participants d’avoir tenu à être présents virtuellement dans ces journées. Votre contribution à l’échange du savoir dans différents domaines de la recherche scientifique sera sans doute d’un grand intérêt pour tout le monde. Merci pour votre confiance. Veuillez consulter le recueil des resumés ci-joint.
Plantes, Molécules Bioactives et Valorisation
l’Ecole Normale Supérieure d'Enseignement Technologique (ENSET) de Skikda
Copyright (c) 2022 https://creativecommons.org/licenses/by-nc/4.0
Première Journée d'Etude Vulnérabilité de la Céréaliculture : Quelles Solutions pour Demain ? (JE-VCQSD-2022)
Préambule La céréaliculture est une composante importante des économies agricole et alimentaire de l’Algérie. Elle couvre en moyenne chaque année, près de 80% de SAU. Cependant, notre production céréalière en est largement déficitaire et est loin de satisfaire la demande d’une population sans cesse croissante à cause d’une agriculture qui fait face à un ensemble de défis. Les risques d’une pression croissante sur les capacités de la fertilité des terres (érosion, désertification, surexploitation des eaux), sur les volumes d'eau disponibles (rabattement des nappes, recours aux eaux fossiles) et sur la qualité des eaux (salinité, pollution) augmentent. De plus, le paysage institutionnel devient plus complexe : le démantèlement d’une grande partie des circuits d’information antérieurs, l’absence d’organisations professionnelles réellement opérationnelles et la défaillance de l’encadrement sectoriel par les normes de qualité ne font que pénaliser les performances de la filière. L’amélioration de la productivité agricole est le seul moyen de lever le verrou du handicap naturel qui caractérise la céréaliculture, et la modernisation des industries agro-alimentaires et son intégration avec le système productif national constituent les voies possibles et nécessaires conduisant à une préservation de la sécurité alimentaire du pays. Veuillez consulter le present recueil de résumés de la Première Journée d'Etude Vulnérabilité de la Céréaliculture : Quelles Solutions pour Demain ? (JE-VCQSD-2022) 1 Décembre 2022 à Tiaret, Algérie.
Evaluation of the nutritional value and antioxidant activity of Opuntia ficus indica seeds in the western region of Algeria
The Opuntia ficus indica (OFI), commonly known as "prickly pear" is a Cactaceae native to Central America that is well suited to arid and semi-arid areas such as Algeria. In recent decades, interest in this long-ignored cactus has increased. Different parts including (cladodes, fruits, flowers) of this plant have been studied, but little work has been devoted to the seeds, which are considered as by-products. It is in this context our research was conducted, it aimed to establish the nutritive value and antioxidant power of these seeds. The study of seed composition showed high crude fiber content estimated at 71.91% and significant levels of fat and protein 10.19% and 9.19%, respectively. The best oil yield was obtained after 8-hour of oil extraction. Given that its density and refractive index were calculated to be 0.916 and 1.470, respectively, this oil is of higher grade. The saponification and acidity were in the range of 161.31 and 1.74, respectively. The total polyphenols were about 1.110 mg EQG / g of DW. The antioxidant activity was revealed by the DPPH free radicals scavenging method. The obtained results were assigned the highest antioxidant activity with flavonoids over 90% at a concentration of 0.5 mg / mL for both fractions (acetate and n -butanol), their EC 50 values are about of 0.089 mg / mL and 0.063 mg / mL, respectively, the latters are close to the EC 50 of ascorbic acid. Thus, OFI seeds may be of interest to the cosmetics industry for its beneficial properties.
Opuntia ficus indica, seeds, oil, nutritive value, antioxidant activity.
Darine KHALDI, Zoubida MAMI, Meriem BELARBI
Assessment of in vitro antioxidant and in vivo anti-inflammatory activities of marine algae from Algerian coast
The search for natural compounds with pharmaceutical activity indicated marine macroalgae as promising sources to supply novel compounds with potential bioactivities. In this study, three algae from the northwest coast of Algeria Sargassum vulgare, Cladostephus hirsutus and Rissoella verruculosa were investigated for their in vitro antioxidant activity and for their in vivo anti-inflammatory activity. Total phenolic, total flavonoid and flavonol, and condensed tannin contents were determined in crude extracts and petroleum ether, ethyl acetate (EA), dichloromethane and butanol fractions, and aqueous residues. Antioxidant activity was evaluated using several in vitro assays: Total Antioxidant Capacity, Ferric Reducing Antioxidant Power and diphenyl picryl hydrazyl radical scavenging activity. Acute toxicity of the crude extract of C. hirsutus was studied in Swiss albino mice and anti-inflammatory activity was evaluated using carrageenan-induced paw edema. Crude extracts revealed high total antioxidant activity ranging from 1.27 to 1.63 mg ascorbic acid equivalent/gram of dry matter for R. verruculosa and C. hirsutus, respectively. For the three algae, EA fractions showed the highest antioxidant activity by the three assays tested, with up to 67% of DPPH radical scavenging activity and an EC50 of 1.114 mg ml-1 by the ferric reducing power assay for the EA fraction of R. verruculosa. No signs of acute toxicity were observed in the crude extract of C. hirsutus at 150 and 300 mg/kg. Significant and dose-dependent activity was shown against acute inflammation with edema reduction of 58.86% and 71.01% at 150 and 300 mg/kg, respectively, after 6 hours of carrageenan administration.
Sargassum vulgare, Cladostephus hirsutus, Rissoella verruculosa, Antioxidant activity, acute toxicity, anti-inflammatory activity.
Mariem BENMAHDJOUB, Khadidja KERZABI-KANOUN
Annual variation in essential oil yield and composition from leaves of Tetraclinis articulata in Northwestern Algeria (Aïn Kebira, Tlemcen Province)
We have been studied the yield and chemical composition of leaf essential oils of Tetraclinis articulata (Vahl) Masters, harvested from the same individual tree in Aïn Kebira of Tlemcen Province (North-western Algeria), at different phenological stages for one year (one sample per month), from March 2014 (full flowering) until February 2015. The twelve essential oil samples obtained by hydrodistillation, were analyzed by GC(RI). The essential oil yields varied drastically from one month to another, ranging between (0.16 to 0.66%) with the most important values observed for December and June. The composition remains qualitatively homogeneous but present quantitative differences. Bornyl acetate (8.6-41.1%), α-pinene (11.1-24.6%) and camphor (2.2-16.4%) were the main compounds in all samples.
Tetraclinis articulata (Vahl) Masters; essential oil; yield, chemical composition, GC(RI), Tlemcen, Algeria.
Date palm “deglet nour” (Phoenix dactylifera) fruit extracts: Functional components, antioxidant, anti-inflammatory activities and gastroprotective effect
Deglet nour, is the most consumed variety of date palm fruit (Phoenix dactylifera L.) in Middle East and North African countries and promote the socio-economic development of growing areas. The fruit contain a high percentage of dietary fibers, salts, vitamins and minerals and the dates are widely used in the traditional medicine to treat a variety of ailments. In order to better discover this natural product, the purpose of this study was to evaluate the phytochemical properties of phenolic rich extract (PRE) and Water-soluble crude polysaccharide extract (WCP) by determining the antioxidant and anti-inflammatory activities and the gastroprotective effect of these extracts against ethanol-induced oxidative stress in rats. The PRE is majority composed by phenolic acids (gallic, ascorbic, ferulic, p-coumaric, caffeic, ellagic, chlorogenic and syringic acids). Chemical analysis of WCP revealed that the extract was composed of 75% carbohydrate. The extracted polysaccharides are principally composed by glucose (49.01 % mol) and fructose (47.21%). Both extracts exhibited high total antioxidant capacity (267 mg GAE/g DR). Polysaccharides are distinguished by a better anti-radical scavenging activity (IC50%=0.5mg/mL) and iron reducing power (EC50%=0.98 mg/mL). The anti-inflammatory activity on macrophages RAW 267.4 showed that Deglet nour extracts decreased the nitric oxide (NO) production at average of 40% at 200µg/mL. Concerning gastroprotective activity, we found that WCP and PRE at lesser degree protected against alcohol-induced volume gastric juice and acidity decrease. More importantly, we showed that Deglet nour extracts and particularly WCP counteracted EtOH-induced gastric lipoperoxidation and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). All these results can justify the utility of Phoenix dactylifera L. as interesting food species with bioactive natural substances that may be used in several fields, such as nutraceuticals and agro-food industry.
Profiling of Minerals in Lemon Savory (Satureja biflora) Traditionally used in the Management of Chronic Wounds
Effective and faster treatment of wounds remains a big challenge facing healthcare professionals and global economies. Diabetic foot ulcers, for example, affect about 15% of all people with diabetes during their lifetime. These ulcers do not respond to conventional therapies, therefore, presenting a high risk for patients such as foot amputation in 85% of the predisposed patients. In addition, the available wound dressing materials are unable to fully address microbial infection, protein adsorption, and elevated amounts of exudates. Although medicinal plants, including lemon savory, have been used by communities worldwide to manage chronic wounds, little data is available especially on the role of metals in topically used formulations. Minerals have demonstrated biocompatibility, capacity to improve cellular processes, and adhesion. The objective of this study was to determine the concentration of manganese, copper, zinc, magnesium, and iron metals in Satureja biflora species, which have been found crucial in wound healing. The leaves of the plant have been used by local Kenyan communities to manage chronic wounds. The plant material was collected from Dondori, Nakuru County, Kenya. The material was then dried and ground into a coarse powder. Upon acid digestion, concentrations of metals of interest were determined using AAS technique. The results indicated that aerial parts of Satureja biflora contain significant quantities of selected metals. Leaves had highest iron concentration (927.49 ± 1.79 ppm) while stems had the lowest magnesium concentration (71.64 ± 0.38 ppm). More studies involving other techniques should be carried out to validate this data for biomimicry applications.
Chronic wounds, Minerals, Satureja biflora
In vitro cytotoxicity of sesquiterpene lactones isolated from Centaurea pullata L. native to Algeria
The phytochemical investigation of the aerial parts of Centaurea pullata L. from Algeria led to the isolation and identification of ten sesquiterpene lactones. The in vitro cytotoxic and antiproliferative potential of the isolated sesquiterpene lactones was tested against fives human cell lines (i.e., DLD1, SF268, MCF7, NCI-H460 and OVCAR3) using the sulforhodamine B (SRB) assay. In total ten compounds were tested, among which the elemanolide melitensin (5) proved the most active demonstrating a growth inhibitory activity below 100 μM against three cell lines NCI-H460, MCF7 and SF268, from the five tested.
Centaurea pullata L., Asteraceae, Sesquiterpene lactone, Melitensin, Cytotoxic activity.
Phytochemical analysis and alpha-amylase inhibitory property of olive (Olea europaea L.) leaves extracts
Evaluation of medicinal plants for their antidiabetic activities has increased considerably around the world as well as in Algeria. Olea europaea, commonly called Zeytoune, is traditionally used by Tlemcen population in Algeria, for treating diabetes mellitus. In this work, we evaluate the capacity of O. europaea leaves crude extracts to inhibit in vitro α-amylase activity. The qualitative phytochemical screening carried out on O. europaea leaves extracts showed the presence of tannins, sterols and triterpenes, saponins, flavonoids and terpenoids. A quantitative analysis of the crude extracts showed significant levels of total polyphenols and flavonoids in hydroacetonic extract, with an amount of 802.57 ± 0.001 mg GAE/ g and 359 ± 0.002 mg CE/ g, respectively. In vitro tests carried out on the inhibitory of α-amylase activity, revealed an inhibitory effects, specifically with hydroacetonic extract in a concentration-dependent manner, with an IC50 value of 0.27 ± 0.02 mg/mL. These findings reveals that O. europaea leaves extracts could represent an interesting source of bioactive compounds and allow to the development of new antidiabetic agents.
Olea europaea; phytochemical screening; antidiabetic; α-amylase
Antihemolytic and antioxidant activities of aerial parts extracts of Zygophyllum album L. and Globularia alypum L. from Algeria
The main intrest of this study was to evaluate the antihemolytic and the antioxidant activities of the aqueous extracts of Globularia alypum and Zygophyllum album prepared by three different methods: decoction, infusion and maceration. Phytochemical tests, total phenolic, flavonoid and tannins contents of the different aqueous extracts were determined. DPPH radical scavenging assay and Ferric Reducing Antioxidant Power (FRAP) were employed for the evaluation of the antioxidant activity. Whereas, the antihemolytic activity was carried out by the evaluation of the protective effect of the various prepared extracts on red blood cells against the radical attack induced by 2,2′-azobis(2-amidinopropane) di-hydrochloride (AAPH). The aqueous extract of Globularia alypum prepared by decoction showed the highest level of total phenolic contents (196.52± 10.76 µg GAE/mg Extract). These findings confirmed the remarkably antioxidant properties of this plant with DPPH scavenging activity and FRAP test. A concentration of 160 µg/ml of this extract showed a protective effect against hemolysis induced by AAPH radical. Hence, Zygophyllum album extracts demonstrated lower level of total phenolic contents (30.41 ± 2.01 µg GAE/mg Extract) and lower antioxidant and antihemolytic activities. Globularia alypum extracts presented a powerful antioxidant and antihemolytic activities especially for the decoction extract whereas Zygophyllum album revealed a weaker protective effect except its maceration extract.
Zygophyllum album; Globularia alypum; phytochemical screening; phenolics compounds; antioxidant; antihemolytic.