Journal of Natural Product Research and Applications <p style="text-align: justify;"><strong>JNPRA </strong>publishes high-quality manuscripts of original works in all aspects of natural compounds of plants, algae, microbes, and animals. The journal is peer-reviewed and publishes two issues per year. Authors are encouraged to submit complete, unpublished, original works that are not under review in any other journals. All submitted manuscripts are subject to initial examination by the editors for the first step and peer-reviewed by at least two anonymous expert referees for the second step. Major topics of JNPRA enclosed are:</p> <ul> <li> <p style="text-align: justify;"><span style="color: #006600;"><strong>Biology and Biological Chemistry:</strong></span> Biochemistry, Biology, Microbiology, Cell and Molecular Biology, Biotechnology, Neuroscience, Phytochemistry.</p> </li> <li> <p style="text-align: justify;"><span style="color: #006600;"><strong>Medicinal Chemistry:</strong></span> Toxicology, Pharmacology, Pharmacognosy, Nutrition and Health, Therapeutics, Bioactive Compounds, Detoxification, Biological activities.</p> </li> <li> <p style="text-align: justify;"><span style="color: #006600;"><strong>Pharmaceutical Formulations: </strong></span>Formulations from natural products, Analysis, Quality Controls.</p> </li> <li> <p style="text-align: justify;"><span style="color: #006600;"><strong>Agricultural and Food Chemistry:</strong></span> Food Technology, Agricultural and Food Safety, Food analysis.</p> </li> <li> <p style="text-align: justify;"><span style="color: #006600;"><strong>Applications of Natural Resources:</strong> </span>Cosmetics, Dietary Supplements, Nutraceuticals, Biopharmaceutical Science, Waste and co-product valuation.</p> </li> <li> <p style="text-align: justify;"><span style="color: #006600;"><strong>Toxicology:</strong> </span>Detoxification, Toxicological medicine, Risk assessment, Safety evaluation, environmental health, Pollution and public health, Waste and co-product valuation.</p> </li> <li> <p style="text-align: justify;"><strong><span style="color: #006600;">Traditional Medicine and Ethnopharmacology</span>:</strong> folkloric medicine, ethnobotany, ethnopharmacology.</p> </li> </ul> <p style="text-align: justify;"><em>All papers that treated the biological activities of crude extracts are accepted after structure elucidation of active molecules. Papers related to In Silico Design of Bioactive Molecules are welcomed for publication. The paper reporting new compounds and manuscripts describing their biological activity are more solicited for publishing in our journal.</em></p> <ul> <li> <p style="text-align: justify;"><span style="color: #663300;"><u><strong>Frequency:</strong> </u></span>The Journal of Natural Product Research and Applications publishes <strong>a twice per year</strong> (April and November).</p> </li> <li style="text-align: justify;"><span style="color: #663300;"><strong>ISSN (online): <em> 2773-4188</em></strong></span></li> <li style="text-align: justify;"><span style="color: #663300;"><strong><em>DOI: <a href="">https://</a></em></strong></span></li> <li> <p style="text-align: justify;"><span style="color: #663300;"><u><strong>Submission:</strong></u> </span>all peer review is single-blind and submissions should be made by sending over our website <a href="">(click to submit).</a></p> </li> <li> <p style="text-align: justify;"><a href=";ouid=102846262700995496353&amp;rtpof=true&amp;sd=true" target="_blank" rel="noopener"><strong><u><span style="color: #663300;">Template JNPRA</span>: </u></strong><span style="color: #000000;">click here to download</span></a></p> </li> <li> <p style="text-align: justify;"><strong><span style="color: #663300;"><u>Journal charges</u></span>:</strong> there are no publication <strong>fees to publish</strong> in the Journal of Natural Product Research and applications (JNPRA).</p> </li> <li> <p style="text-align: justify;"><strong><span style="color: #663300;"><u>Format:</u></span> </strong>Currently, the Journal of Natural Product Research and Applications is publishing<strong> online-only</strong> format.</p> </li> <li> <p style="text-align: justify;"><strong><span style="color: #663300;"><u>Manuscript categories:</u></span> </strong>the Journal of Natural Product Research and Applications publishes <em>full Papers, short communications, reviews, letters to the editor, notes, and book reviews.</em></p> </li> </ul> University of Abou Bekr Belkaid at Tlemcen en-US Journal of Natural Product Research and Applications 2773-4188 Antioxidant and cytotoxic activities of extracts from Haloxylon scoparium Pomel <p><em>Haloxylon scoparium </em>Pomel is a plant known to possess anti-cancer properties as well as anti-plasmodial and larvicidal activities. The aerial parts are used in traditional medicine to treat disorders and problems of the eye and vision, digestion problems, dermatoses, scorpion pitting. The antioxidant and anticancer activities of different extracts (dichloromethane, ethyl acetate and butanol) from flowers and stems were studied. The flowers exhibited strong antioxidant activity using the oxygen radical absorbance capacity method and a cell-based assay. The most interesting results are obtained with the ethyl acetate extracts of the flowers active with an ORAC value of 6.6 μmol TE / mg, followed by the butanol extract of the same organ (ORAC value of 6.22 μmol TE/ mg). The experiments carried out using the cellular antioxidant protocol reveal a very strong antioxidant activity, particularly for the ethyl acetate and butanol extract of the flowers with an IC<sub>50</sub> value of 0.029 ± 0.004 µg/ mL and 0.3 ± 0.1 µg/ mL respectively . The cytotoxic activity (Resazurin tests) was found to be moderate against A-549 (lung carcinoma) and DLD-1(colon carcinoma) cells, with IC<sub>50</sub> values of 110 ± 13 and 117 ± 14 μg/ mL, respectively. All the results obtained constitute a scientific justification for the use traditional of the plants studied and once again confirms the relevance of the remedies traditional in the treatment of many diseases.</p> Samira BELHADJ TAHAR André PICHETTE, Jean LEGAULT Mahfoud HADJ-MAHAMMED Copyright (c) 2024 2024-02-13 2024-02-13 3 02 1 10 10.46325/jnpra.v3i02.60 Phytochemical study and evaluation of α-Amylase inhibitory effect of extracts from Cistanche violacea Desf. <p><em>Cistanche violacea</em> Desf. is a species of the family Orobanchaceae. It is an angiosperm dicotyledonous holoparasitic plant, which lacks chlorophyll. This work focuses on the search for an inhibitory effect of the enzymatic activity of α-amylase, in the presence of the crude water-methanol extract, of the underground part of <em>C. violacea</em>, prepared by maceration or decoction and its ethyl acetate fractions. Qualitative phytochemical tests and quantitative assays of phenolic compounds of the prepared extracts were performed. The effect of each extract on the activity of the α-amylase enzyme was evaluated under optimal conditions (pH 6.9 and T° 37°C). The results of phytochemical tests revealed the presence of tannins, flavonoids, free quinones, terpenoids and reducing compounds. The determination of phenolic compounds showed that the ethyl acetate extract prepared by maceration was the richest in total polyphenols and flavonoids with contents of the order of 82.937±6.750μg EqGA/mg D.W and 112.345±25.719μg EqC/mg D.W respectively. While the crude hydromethanolic extract prepared by maceration presented the highest content of condensed tannins (525.5±77.5μg EqC/mg D.W). On the other hand, the ethyl acetate extract prepared by decoction of the underground part of <em>C. violacea</em> showed a better inhibitory activity of the α-amylase enzyme, with IC<sub>50</sub> value of 1.88 mg/ml, compared to the other extracts tested. This activity remains low compared to acarbose (IC<sub>50</sub> = 0.132 mg/ml). We concluded that the roots of <em>C. violacea</em> exerts an inhibitory effect on α-amylase activity and thus on the regulation of hyperglycemia through a decrease in intestinal glucose absorption</p> Ibrahim BOUCHEKIF Rachid AZZI ABBOU Fayza Brahim BABALI Kouider BOUDAOUD Copyright (c) 2024 2024-02-13 2024-02-13 3 02 11 22 10.46325/jnpra.v3i02.62 Maceration, microwave assisted and ultrasound assisted extraction of phenolic compounds from Tunisian Limoniastrum monopetalum: kinetic modeling and chemical analysis <p>Maceration, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE) were investigated in this study, focusing on the selectivity towards phenolic compounds in ethanol extracts from <em>Limoniastrum monopetalum</em> leaves using the Folin-Ciocalteu method and kinetic models of extractions. Moreover, several phenolic compounds were identified by RP-HPLC. Results showed that total polyphenol compounds (TPC) under the MAE conditions (ethanol, 700 W microwave power, 60 s extraction time, and 1 mL/g solvent-to-solid ratio) were 75.707 mg GAE/100 g dried sample in only one minute of extraction. We conclude that MAE is a promising extraction method for polyphenols. Model results were correlated with mathematical models of the extraction process. Six phenolic acids and three flavonoids were identified by HPLC–DAD. MAE is a valuable and green analytical methodology for the investigation of phenolic components in natural plants.</p> Olfa Khedher Manel Elakremi Ridha Ben Salem Younes Moussaoui Copyright (c) 2024 2024-02-13 2024-02-13 3 02 23 33 10.46325/jnpra.v3i02.63 In vitro antioxidant and antidiabetic activities of the hydro-acetone extracts of Ricinus communis and Teucrium polium from Tlemcen area <p>The aim of the present study was to determine total phenolic and total flavonoid contents in hydro-acetone extracts from the roots of <em>Ricinus communis </em>and the aerial part of <em>Teucrium polium</em>, and to determine <em>in vitro </em>their antioxidant and antidiabetic activities using DPPH, FRAP, and α-amylase activity assay, respectively. The obtained results reveled that <em>R. communis </em>roots hydro-acetone extract contains high contents of polyphenols (499.55 ± 0.02 µg GAE/mg DE) and flavonoids (28.21 ± 0.01 µg Cat eq/mg DE) than <em>T. polium</em> aerial part extracts. Both plant extracts showed <em>in vitro</em> antioxidant and antidiabetic activities, where <em>R. communis </em>showed a potent free radical scavenging activity (DPPH IC<sub>50</sub>=2.01 ± 0.01 µg/mL) and high iron reduction capacity (FRAP EC<sub>50</sub>=41.32 ± 0.01 µg/mL) more than <em>T. polium</em> extract (DPPH IC<sub>50</sub>= 7.40 ± 0.01 µg/mL; FRAP EC<sub>50</sub>=74.63 ± 0.01 µg/mL). In addition, in α-amylase activity assay <em>T. polium </em>was more efficient in inhibiting this enzyme (EC<sub>50</sub>= 924 ± 0.19 µg/mL) compared to <em>R. communis</em> extract (EC<sub>50</sub>= 1300 ± 0.03 µg/mL).</p> Nabila BENARIBA, Zohra FEKHIKHER, Radia BRIXI-GORMAT, Hanane BENRAMDANE, Houda YOUBI , Ibtissam KOUAR , Hanane MILOUDI , Soumia MEHARRAR Imad Abdelhamid EL HACI Houria MEDJDOUB , Rachid AZZ Copyright (c) 2024 2024-02-13 2024-02-13 3 02 34 46 10.46325/jnpra.v3i02.64 Phycochemistry study and antimicrobial activity of Spirogyra freshwater green microalgae from Algeria <p>In Algeria, algae have not been adequately explored for their potential as a source of bioactive substances. In this context the blooms of green alga <em>Spirogyra</em> sp. were collected from freshwater habitats in Oued El Mellah near M’sila province of Algeria. Hexane, acetone and ethanol crude extracts were screened for antimicrobial activity against <em>Staphylococcus aureus</em>, <em>Listeria monocytogenes</em>, <em>Pseudomonas aeruginosa</em>, <em>Escherichia coli</em>, <em>Salmonella typhi</em> and <em>Candida albicans</em>. The contents of total soluble sugars, soluble proteins, total lipids and phenolic acids were also determined. The present findings revealed that the highest extraction yield was obtained from ethanol (4.93%) compared to acetone (3.67%) and hexane (2.33%).&nbsp;The contents of phycochemical compounds were 32.5% of soluble carbohydrates, 15.84% of lipids, 15.6% of phenolic acids and 5.16% of proteins. All extracts exhibited better and stronger antibacterial activities against the five pathogenic bacteria with inhibition zones diameter ranged from 13.5-29 mm for acetone extract, from 13-25 mm for ethanol extract and from 12.5-20.5 mm for hexane extract at 7.5 µL. The minimal inhibitory concentration (MIC) was in the range of 15-40 mg mL<sup>-1</sup>. The acetone extract was more effective than others extracts with MIC value of 15 mg mL<sup>-1</sup> for all bacteria. These findings indicate the presence of promising antibacterial compounds originating from microalgae, which can be exploited for the production of new antimicrobial agents.</p> Larbi BELYAGOUBI Rachid CHAIBI, Hicham GOUZI Fatima Zohra AISSAOUI, Zahrat El Oula BENAMAR Nabila BELYAGOUBI-BENAHMMOU Copyright (c) 2024 2024-02-13 2024-02-13 3 02 46 60 10.46325/jnpra.v3i02.65