Comparison of the biological power of a medicinal plant: Eucalyptus globulus obtained from two regions of Algeria (Tizi-Ouzou/Blida).
DOI:
https://doi.org/10.46325/nd79bv04Keywords:
Eucalyptus globulus, Blida, Tizi-Ouzou, essential oil, antioxidant activity, , antibacterial activityAbstract
Eucalyptus globulus, a species of the Myrtaceae family and a common medicinal plant in traditional Algerian medicine, is already known for its biological properties and especially its antibacterial and antioxidant properties. Ecological conditions influence pharmacological activity; Eucalyptus is therefore an interesting species to study. This work consists of evaluating the biological activity of methanolic extracts and essential oils of E. coli leaves. Globulus was collected from two regions in northern Algeria: Blida and Tizi-Ouzou. A qualitative phytochemical screening was carried out on the methanolic extracts followed by a quantitative analysis of the polyphenol and flavonoid contents. Antioxidant activity was assessed using the DPPH free radical scavenging assay, and antibacterial activity by the agar diffusion method. Phytochemical analysis revealed a qualitative diversity in the composition of extracts between the two regions. Indeed, extracts from Blida showed higher levels of phenols and flavonoids than those from Tizi-Ouzou. The antibacterial activity of the extracts was found to be high, while the antioxidant activity varied according to geographical origin. This reveals an influence of geoclimatic conditions on the antioxidant power. The results obtained reveal the effect of geographical origin on the chemical composition and biological activities of Eucalyptus globulus. These variations must be taken into account in the phytotherapeutic development of the species.
References
Akin M., Aktum A., Nostro A., (2010). Antibacterial activity and composition of the essential oils of Eucalyptus camaldulensis Dehn. And Myrtus communis L. growing in Northern Cyprus. African Journal of Biotechnology, Vol. 9 (4), p: 531-535, 25 ISSN 1684-5315.
Aprotosoaie A. C., Spac A. D., Hancianu M., Miron A., Tanasescu V. F., Dorneanu V., Stănescu U., (2010). The chemical profile of essential oils obtained from fennel fruits (Foeniculum vulgare Mill.). Farmacia; 58 (1), p:46-54.
Arias M. E., Gomez J. D., Cudmani N. M., Vattuone M. A., Isla M. I., (2004). Antibacterial activity of ethanolic and aqueous extracts of Acacia aroma Gill. Life Sciences, 75 p: 191-202.
Belyagoubi L., (2006). Effet de quelques essences végétales sur la croissance des moisissures de détérioration des céréales. Mémoire de magister. Université Abou Bekr Belkaid.
Benini C., (2007). Contribution à l'étude de la diversification de la production d'huiles essentielles aux Comores. Engineering thesis. Gembloux University.
Bharti P., Bai S. S., Seasotiya L., Malik A., Dalal S., (2012). Antibacterial activity and Chemical Composition of Essential Oils of Ten Aromatic Plants against selected Bacteria. International Journal of Drug Development & Research. Vol. 4. Issue 4. ISSN 0975-9344.
Brand-Williams, W., Cuvelier, M. E., Berset, C., (1995). Use of a free radical method to evaluate antioxidant activity. LWT – Food Science and Technology, 28(1), 25-30.
Bougandoura N., Bendimerad N., (2013). Evaluation de l’activité antioxydante des extraits aqueux et méthanolique de Satureja calamintha ssp. Nepeta (L.) Briq. Nature & Technologie, Volume 5. Numéro 2, p: 14-19.
Boukhatem M. N., Ferhat M. A., Kameli A., Saidi F., Kerkedi W., Sadok Bouziane M., (2014). Quality assessment of the essential oil from Eucalyptus globulus Labill of Blida (Algeria) origin. Int Lett of Chem, Phys and Astron.17(3): 303-15.
Boukhatem M. N., Ferhat M. A., Kameli A., Mekarnia M., (2017). Eucalyptus globulus (Labill.) : un arbre à essence aux mille vertus. Phytothérapie. 2 : 203-214.
Bruneton J., (1999). Pharmacognosy: phytochemistry, medicinal plants. 3rd edn, Tec & Doc. Lavoisier, Paris.
Cadahía E., E. Conde E., García-Vallejow M. C., Fernández de Simón B., (1997). High Pressure Liquid Chromatographic Analysis of Polyphenols in Leaves of Eucalyptus camaldulensis, E. globulus and E. rudis: Proanthocyanidins, Ellagitannins and Flavonol Glycosides. Phytochemical Analysis, 8, p: 78-83.
Cardoso J. C., Oliveira M. E. BS., Cardoso F.CL., (2019). Advances and challenges on the in vitro production of secondary metabolites from medicinal plants. Horticultura Brasileira. 37(2), p :124-132.
Chowdhury J. U., Mobarok Md. H., Bhuiyan Md. N. I., Nandi N. C., (2009). Constituents of essential oils from leaves and seeds of Foeniculum vulgare Mill. Cultivated in bangladesh. Bangladesh J. Bot. chrysanthemum trifurcatum (asterarceae). Doctoral thesis in Applied Biochemistry, p: 169- 178.
Close D. C., Mc Arthur C., Pietrzykowski E., Fitzgerald H., Paterson S., (2004). Evaluating effects of nursery and post-planting nutrient regimes on leaf chemistry and browsing of eucalypt seedlings in plantations. Forest Ecology and Management, 200, p: 101-112.
Daker M., Abdullah N., Vikineswary S., Goh P. C., Kuppusamy U. R., (2008). Antioxidant from maize and maize fermented by Marasmiellus sp. as stabilizer of lipidrich foods. Food Chemistry, 107, p: 1092-1098.
Damiani N., Fernández N. J., Porrini M. P., Gende L. B., Álvarez E., Buffa F., Brasesco C., D. Maggi M. D., Marcangeli J. A., Martín J. Eguaras M. J. (2013). Laurel leaf extracts for honeybee pest and disease management: antimicrobial, and acaricidal activity. Journal Parasitology. 113, p:701-709.
Dastmalchi K., Damien Dorman H. J., Oinonen P. P., Darwis Y., Laakso I., Hiltunen R., (2008). Chemical composition and in vitro antioxidative activity of lemon balm (Melissa officinalis L.) extract. LWT, 41, p: 391-400.
De Billerbeck V.-G., (2007). Essential oils and antibiotic-resistant bacteria. Phytotherapie, 5, p: 249-253.
Djeridane A., Yousfi M., Nadjemi B., Boutassouna D., Stocker P., Vidal N., (2006). Antioxidant activity of same Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97, p: 654-660.
Ebrahimi N. S., Hadian J., Mirjalili M. H., Sonboli A., Yousefadi M., (2008). Essential oil composition and antibacterial activity of Thymus caramanicus at different phenological stages. Food Chemistry, Volume 110, Issue 4, p: 927-931.
Elaissi A., Medini H., Simmonds M., Lynen F., Farhat F., Chemli R., (2011). Variation in volatile leaf oils of seven Eucalyptus species Harvested from Zerniza Arboreta (Tunisia). Chem Biodivers, 8, p: 362-72.
Filippi J. J., Lanfranchi D. A., Prado S., Baldovini N., Meierhenrich U. J., (2006). Composition, distribution and antibacterial activity of the essential oil of Achillealigustica All. From Corsica. - J. Agric. Food Chem. 54 ; p: 6308-6313.
Garnero J., (1991). Les huiles essentielles, leur obtention, leur composition, leur analyse et leur normalisation. Ed Encyclopédie des médecines naturelles, Paris, France.
Ghedadba N., Bousselsla H., Hambaba L., Mouloud Y., (2014). Evaluation de l’activité antioxydant et antimicrobienne des feuilles et des sommités fleuries de Marrubium vulgare L. Phytothérapie, 12(1), p :15-24.
Guo Q.-M., Yang X.-W., (2006). Cypellocarpin C and other compounds from the fruits of Eucalyptus globulus Labill. Biochemical Systematics and Ecology, Volume 34, Number 6, p. 543-545.
Gutiérrez M. E., Garcia A. F., de Madariaga M. A., Sagrista M. L., Casado F. J., Mora M., (2003). Interaction of tocopherols and phenolic compounds with membrane lipid components: Evaluation of their antioxidant activity in a liposomal model system. Life Science, 72, p: 2337-2360.
Hazzit M. B., (2015). Composition chimique et activité antimicrobienne de l’extrait non volatil et des huiles. Recherche Agronomique, (27), p: 118-129.
Hogg J. W., Terhune S. J., Pichitakul N., (1972). Essential oils and theirconstituents. IX. The oils of Ocimum sanctum and Ocimum basilicum from Thailand. FlavorInd, Jan. 3, p: 47-49.
Karou D., Dicko M. H., Simpore J., Traore A., (2005). Antioxidant and antibacterial activities of polyphenols from ethnomedicinal plants of Burkina Faso. African Journal of Biotechnology, 4, p: 823-828.
Khan R., Islam B., Akram M., Shakil S., Ahmad A., Siddiqui M., Khan U., (2009). Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Molecules, 14, p: 586-597.
Lamaison J. L. C., Carnet A., (1990). Teneurs en principaux falvonoïdes des fleurs et des feuilles de Crataegus monogyna Jacq. et de Crataegus laevigata (Poiret) DC. en fonction de la période de végétation. Pharm Acta Helv 65, p: 315-20.
Lee K.W., Kim Y. J., Lee H. J., Lee C.Y., (2003). Cocao has more phenolic phytochemicals and a higher antioxidant capacity thane as and redwine. Food chemistry. 51, p: 7292-7295.
Lee B., Lee S., Lee H., Sim G., Kim J., Cho Y., Hong, J., (2007). Antioxidative and Photo-protective Effects of Coumarins Isolated from Fraxinus chinensis. Arch Pharm Res, 30, p: 1293-1301.
Li B., Cheng W., Wong W., Jiand Y., (2003). Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food chemistry, 102, p:771-776.
Luís-A A., Duarte A., Gominho-b J., Domingues-A F., Paula-Duarte A., (2016). Chemical composition, antioxidant, antibacterial and anti-quorum sensing activities of Eucalyptus globulus and Eucalyptus radiata essential oils. Industrial Crops and Product.79, p: 274-283.
Macheix J. J., Fleuriet A., Jay-Allemand C. (2005). Les composés phénoliques des végétaux : Un exemple de métabolites secondaires d’importance économique. PPUR presses polytechniques. Pp 116.
Maisuthisakul P., Pasuk S., Ritthiruangdej P., (2008). Relationship between antioxidant properties and chemical composition of some thay plants. Journal of Food Composition and Analysis, 21, p: 229-24.
Martins M. D. R., Arantes S., Candeias F., Tinoco M. T., Cruz-Morais J., (2014). Antioxidant, antimicrobial and toxicological properties of Schinusmolle L. essential oils. J. Ethnopharmacol, 151, p: 485-492.
Marzoukia H., Elaissi A., Khaldi A., Bouzid S., Falconieri D., Marongiu B., Piras A., Porcedda S., (2009). Seasonal and Geographical Variation of Laurus nobilis L. Essential oil from Tunisia. The Open Natural Products Journal, 2, p: 86-91.
Miliauskas G., Venskutonis P. R., Van Beek T. A., (2004). Screening of radical scavenging activity of some medicinal and aromatic plant extract. Food chemistry, 85, p: 231-237.
Mohammedi Z., (2006). Etude du pouvoir antimicrobien et antioxydant des huiles essentielles et des flavonoïdes de quelques plantes de la région de Tlemcen. Magistère thesis. Université Abou Bakr Belkaid Tlemcen.
Mulyaningsih S., Sporer F., Zimmermann S., Reichling J., Winka M., (2010). Synergistic properties of the terpenoids aromadendrene and 1,8-cineole from the essential oil of Eucalyptus globulus against antibiotic-susceptible and antibiotic-resistant pathogens. Phytomedicine 17, p: 1061-1066.
Natarajan D., John Britto S., Srinivasan K., Nagamurugan N., Mohanasundari C., Perumal G., (2005). Antibacterial activity of Euphorbiafusiformis-A rare medicinalherb. J-Ethnopharmacol. 102, p: 123-126.
Olayinka A. J., Olawumi O. O., Olalekan A.M., Abimbola A. S., Idiat I.D., Theophilus O. A., (2012). Chemical composition, antioxidant and cytotoxic effects of Eucalyptus globulus grown in north-central Nigeria. J. Nat. Prod. Plant Resour, 2(1), p:1-8.
Oliveira I., Soussa A., Morais J. S., Ferreira I. C. F. R., Bento A., Estevinho L., Pereira J.A., (2008). Chemical composition, and antioxidant and antimicrobial activities of three hazelnut (Corylus avellana L.) cultivars. Food and Chemical Toxicology, 46, p: 1801-1807.
Olle M., Bender I., (2010). The content of oils in Umbelliferous crops and its formation. Agronomy Research 8 (Special Issue III), p: 687-696.
Oumaskour K., Boujaber N., Etahiri S., Assobhei O., (2012). Screening of antibacterial and antifungal activities in green and brown algae from the coast of Sidi Bouzid (El Jadida, Morocco). African journal of Biotechnology, 11(104), p: 16831-16837.
Pereira S., Freire S. R. C., Neto P., Silvestre J. D., Silva M. S. A., (2005). Chemical composition of the essential oil distilled fromthe fruits of Eucalyptus globulus grown in Portugal. Flavour and fragrance journal flavour fragr. J.; 20, p: 407-409.
Pinto E., Pina-Vaz C., Salgueiro L., Goncalves M. J., Costa De-Oliveira S., Cavaleiro C., (2006). Antifungal activity of the essential oil of Thymus pulegioides on Candida Aspergillus and dermatophyte species. J of Med Microbiol. 55, p: 1367-73.
Recoquillay F., (2009). L’intérêt des huiles essentielles. 9ème journée productions porcines et avicoles. P : 59.
Richard H., Peyron F., (1992). Epices et aromates. Ed. Tec & Doc-Lavoisier. Paris. P :339.
Roberto G., Baratta M.T., (2000). Antioxidant activity of selected essential oil component in two lipid model systems. Food Chem; 69(8), p:167-74.
Salminen J.-P.; Ossipov V.; Loponen J.; Haukioja E., Pihlaja K., (1999). Characterisation of hydrolysable tannins from leaves of Betula pubescens by high-performance liquid chromatography-mass spectrometry. J. Chromatogr A., 864, p: 283-291.
Salminen J.-P., Roslin T., Karonen M., Sinkkonen J., Pihlaja K., Pulkkinen P., (2004). Seasonal variation in the content of hydrolyzable tannins, flavonoid glycosides, and proanthocyanidins in oak leaves. Journal of Chemical Ecology, 30, p: 1693-1711.
Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., Yoga Latha, L. (2011). Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African Journal of Traditional, Complementary and Alternative Medicines, 8(1), 1–10. https://doi.org/10.4314/ajtcam.v8i1.60483
Seladji M., (2015). Etude phytochimique, activités antioxydante et antimicrobiennes des extraits de cinq plantes médicinales et analyse de leurs huiles essentielles. Thèse de doctorat en biologie cellulaire et biochimie. Université Abou Bekr Belkaid Tlemcen.
Silva M. S., Abe S. Y., Murakami F.S., Frensch G., Marques F.A., Nakashima T., (2011). Essential Oils from Different Plant Parts of Eucalyptus cinerea F. Muell. ex Benth. (Myrtaceae) as a Source of 1,8-Cineole and Their Bioactivities. Pharmaceuticals (Basel), 4(12), p: 1535-1550.
Singleton V. L., Orthofer R., Lamuela-Raventós R. M., (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, volume 299, p: 152-178.
Traore N., Sidibe L., Bouare S., Harama D., Somboro A., Fofana B., Diallo D. Figueredo G., Chalchat J. C., (2013). Activités antimicrobiennes des huiles essentielles de Eucalyptus citriodora Hook et Eucalyptus houseana W. Fitzg. Ex Maiden. Int. J. Biol. Chem.Sci. 7(2), p : 800-804, ISSN 1991-8631.
Tyagui A., Malik A., (2011). Antimicrobial potential and chemical composition of Eucalyptus globulus oil in liquid and vapour phase against food spoilage microorganisms. Food Chemistry, 126, p: 228-235.
Ulanowska K., Majchrzyk A., Moskot M., Jakbkiewicz-Banecka J., Âgrzyn G., (2007). Assessment of antibacterial effects of flavonoids by estimation of generation times in liquid bacterial cultures. Biologia, 62, p: 132-135.
Vankar P. S., Tiwari V., Srivastava J., (2006). Extracts of stem bark of Eucalyptus globulus as food dye with antioxidant properties. Electronic Journal of Environmental, Agricultural and Food Chemistry, 5, p: 1664-1668.
Yang Y. C., Choi H. Y., Choi W. S., Clark J. M., Ahn Y. J., (2004). Ovicidal and adulticidal activity of Eucalyptus globulus leaf oil terpenoids against Pediculus humanus capitis (Anoplura : Pediculidae). J Agric Food Chem. 52(9): 2507-11.
Zin Z. M., Hamid A., Osman A., Saari N., (2006). Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda). Food Chemistry, Volume 94, Issue 2, p: 169-178.
Zrira S., El khiran F., Benjllal B., (1994). Huiles essentielles de six espèces xérophyles d’Eucalyptus : effet du milieu sur les rendements et la composition-chimique. Actes Inst. Agron. Veto (Maroc), Vol. 14 (l), p: 5-9.
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