Tumor and redox markers in colorectal cancer and correlation between levels of nitric oxide, superoxide anion, malondialdehyde and cancer antigens « CEA and CA 19-9 »
Keywords:
Colorectal cancer - Oxidative stress - Catalase - Glutathione - Dietary intake - Tumour markers.Abstract
Introduction : Free radicals have long been known to play a determining role in carcinogenesis. The objectives of this study were to assess the food profile, physical activity and redox status. The correlation between .NO, O2.- and MDA and tumour markers were investigated.
Methods: Dietary frequency was carried out by using an alimentary chequebook. A basic questionnaire was presented to subjects to obtain individual data. Biochemical and oxidative stress markers were measured by spectrophotometry.
Results : The obtained results showed that BMI values were significantly lower in CRC patients compared to controls (p < 0.01), i-e., 24,53 ±3,69 vs 21,77 ± 4,64 kg/m2. Physical activity revealed a significant reduction (p < 0.05) in both sexes of CRC, i-e., 116,25 ± 23,03 vs 96,37 ± 16,1METs. Food frequency underlined that milk and white meat were higher in male CRC cases in contrary of red meat which was lower (p < 0.05). In female CRC, read meat and oilseeds were less consummed (p < 0.05).Significant low blood suggar (p < 0.001) and albumin (p < 0.01) were recorded in CRC cases. Cholesterol and triglycerides were higher in CRC cases (p < 0.01). ROS concentrations were significantly higher (p < 0.001) at the contrary of antioxidant levels which were markedly lower in CRC cases (p < 0.01). The MDA, O2-. , .NO were relevantly correlated to ECA and CA 19-9.
Conclusion : There is an evident imbalance in the redox status. The latter is linked to the nutritional profile and physical activity. The obtained correlations open up new research avenues.
References
– Acevedo-Leon D, Angel S, Abril G, Sanz-García P, Estan-Capell N, Banuls C et al. (2023). The role of oxidative stress, tumor and inflammatory markers in colorectal cancer patients: A one-year follow-up study. Redox Biology 62, 102662. https://doi: 10.1016/j.redox.2023.102662
– Aebi H (1974). Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, 2nd edn. Verlag Chemie GmbH, Weinheim. /NewYork 673-680. https://doi.10.1016/b978-0-12-091302-2.50032-3
– Ainsworth BE, Haskell WL, Whitt MC, Irwin ML, Swartz AM, Strath SJ et al. (2000). Compendium of physical activities: an update of activity codes and MET intensities. Med Sci Sports Exerc 32(9): S498–S504, 2000. https://doi: 10.1097/00005768-200009001-00009
– Althumairi A, Lazarev MG, Gearhart SL (2016). Inflammatory bowel disease associated neoplasia: A surgeon’s perspective. World J Gastroenterol 21; 22(3): 961–973. https://doi: 10.3748/wjg.v22.i3.961
– Badid N1, Baba Ahmed FZ, Merzouk H, Belbraouet S, Mokhtari N, Merzouk SA, Benhabib R, Hamzaoui D, Narce M (2010). Oxidant/Antioxidant Status, Lipids and Hormonal Profile in Overweight Women with Breast Cancer. Pathol. Oncol. Res 16, 159–167. https://doi: 10.1007/s12253-009-9199-0
– Badid N1, Merzouk H, Charef A (2021).Assessment of Blood Antioxidant Defense and Oxidative Stress in Colorectal Carcinoma Patients Undergoing Capecitabine and Oxaliplatin Combined with Bevacizumab Treatment. Electronic Physician 13, (1) : 7822-7829. https://doi: 10.19082/7822
– Badid N1, Merzouk H, Meguenni K, Charef A, Hamzaoui D (2020). Clinical interests of the study of adaptive oxidative / nitrosative stress in breast and ovarian cancer before and under chemotherapy– A case control Study- Medical Technologies Journal 4 (2) : 556-557. https://doi.org/10.26415/2572-004X-vol4iss2p556-557
– Bandara N, Gurusinghe S, Yong Lim S, Chen H, Chen S, Wang D et al. (2016). Molecular control of nitric oxide synthesis through eNOS and caveolin-1 interaction regulates osteogenic differentiation of adipose-derived stem cells by modulation of Wnt/β-catenin signaling. Stem Cell Res Ther 7;7(1):182. https://doi.org/10.1186/s13287-016-0442-9
– Bardelcikova A, Šoltys J, Mojžiš J (2023). Oxidative Stress, Inflammation and Colorectal Cancer: An Overview. Antioxidants (Basel) 12(4): 901. https://doi: 10.3390/antiox12040901
– Cao S, Chen C, Gu D, Wang Z and Xu G (2023). Establishment and external verification of an oxidative stress related gene signature to predict clinical outcomes and therapeutic responses of colorectal cancer. Front. Pharmacol 13:991881. https://doi: 10.3389/fphar.2022.991881
– Chan CW, Lee PH (2016).Association between dietary fibre intake with cancer and all-cause mortality among 15 740 adults: the National Health and Nutrition Examination Survey III. J Hum Nutr Diet 29(5):633-42. https://doi: 10.1111/jhn.12389
– Desnoyers A, Riesco E, Fülöp T, Pavic M (2016).Physical activity and cancer. Rev Med Interne 37(6):399-405. doi: 10.1016/j.revmed.2015.12.021
– Ellman GL (1959). Tissue sulfhydryl groups. Archives of biochemistry and biophysics 82(1), 70-77. https://doi: 10.1016/0003-9861(59)90090-6
– Fanali G, di Masi A, Trezza V, Marino M, Fasano M, Ascenzi P (2012). Human serum albumin: From bench to bedside. Molecular Aspects of Medicine 33(3): 209-290. https://doi: 10.1016/j.mam.2011.12.002
– Gagnière J, Raisch J, Veziant J, Barnich N, Bonnet R, Buc E et al (2016). Gut microbiota imbalance and colorectal cancer. World J Gastroenterol 4;22(2):501-18. https://doi: 10.3748/wjg.v22.i2.501
– Goldberg DM, Spooner RJ (1992). Glutathione reductase. In: Bergmeyer HB (ed) Methods of enzymatic analysis, vol 3, 3rd ed. Verlag Chemie GmbH, Weinheim pp 258–265. https://doi: 10.1016/S0031-3025(16)36756-3
– Ighodaro OM, Akinloye OA (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): their fundamental role in the entire antioxidant defence grid, Alexandria Med J 54 : 287–293. https://doi.org/10.1016/j.ajme.2017.09.001
– INSP : Institut national de santé publique (2021). Colorectal Cancer- National Institute of Public Health. Algeria. www.aps.dz/sante-science-technologie/119842. https://www.insp.dz
– Jacotot B, Campillo B (2003). Nutrition humaine. Masson. Paris, 311p. ISBN : 2-294-00988-6
– Lech G, Słotwiński R, Słodkowski M, Krasnodębski IW (2016). Colorectal cancer tumour markers and biomarkers: Recent therapeutic advances. World J Gastroenterol 7;22(5):1745-55. https://doi: 10.3748/wjg.v22.i5.1745
– Hauner D, Rack B, Friedl T, Hepp P, Janni W, Hauner H (2020). Rationale and description of a lifestyle intervention programme to achieve moderate weight loss in women with non-metastatic breast cancer: the lifestyle intervention part of the SUCCESS C Study. BMJ Nutr Prev Health. 3(2) : 213–219. https://doi: 10.1136/bmjnph-2020-000119
– Muka T, Kraja B , Ruiter R , de Keyser CE , Hofman A , Stricker BH , Kiefte-de Jong JC, Franco OH (2016). Dietary polyunsaturated fatty acids intake modifies the positive association between serum total cholesterol and colorectal cancer risk: the Rotterdam Study. J Epidemiol Community Health 70(9):881-7. https://doi: 10.1136/jech-2015-206556
– Paglia DE, Valentine WN (1967). Studies on the quantitative and qualitative characterizations of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169. PMID: 6066618
– Pasupathi P, Saravanan G, Chinnaswamy P, Bakthavathsalam G (2009).Effect of chronic smoking on lipid peroxidation and antioxidant status in gastric carcinoma patients. Indian J Gastroentero. 28(2):65-7. https://doi: 10.1007/s12664-009-0021-4
– Patil SB, Kodliwadmath MV, Kodliwadmath SM (2008). Correlation between lipid peroxidation and non-enzymatic antioxidants in pregnancy induced hypertension. Indian Journal of Clinical Biochemistry 23(1): 45-48. https://doi: 10.1007/s12291-008-0011-9
– Piecuch A, Kurek J, Kucharzewski M, Wyrobiec G, Jasinski D, BrzozowaZasada M (2020). Catalase immunoexpression in colorectal lesions, Przeglad Gastroenterol 15 : 330–337. https://doi:10.5114/pg.2020.101562
– Radišauskas R , Kuzmickienė I, Milinavičienė E, Everatt R (2016).Hypertension, serum lipids and cancer risk: A review of epidemiological evidence. Medicina (Kaunas). 52(2):89-98. https://doi: 10.1016/j.medici.2016.03.002
– Ramirez M, Rajaram S, Steininger RJ, Osipchuk D, Roth MA, Morinishi LS, et al (2016). Diverse drug-resistance mechanisms can emerge from drug-tolerant cancer persister cells. Nat Commun 19: 7 : 10690. https://doi: 10.1038/ncomms10690
– Roe JH, Kuether CA (1943). The determination of ascorbic acid in whole blood and urine through the 2, 4-dinitrophenylhydrazine derivatives of dehydroascorbic acid. J Biol Chem 147:399–407. https://doi.org/10.1016/S0021-9258(18)72395-8
– Song Y, Liu M, Yang FG, Cui LH, Lu XY, Chen C (2015). Dietary fibre and the risk of colorectal cancer: a case- control study. Asian Pac J Cancer Prev 16(9): 3747-52. https://doi: 10.7314/apjcp.2015.16.9.3747
– Ten Kate M, Van der Wal JBC, Sluiter W, Hofland LJ, Jeekel J, Sonneveld P, Van Eijck CHJ (2006).The role of superoxide anions in the development of distant tumour recurrence. British Journal of Cancer 95, 1497–1503. https://doi: 10.1038/sj.bjc.6603436
– Useros JM, Foncillas JG (2016). Obesity and colorectal cancer: molecular features of adipose tissue. J Transl Med 2016; 14: 21. https://doi: 10.1186/s12967-016-0772-5
– Wang CD , Sun Y, Chen N ,Huang L , Huang JW, Zhu M , Wang T, Ji YL (2016). The Role of Catalase C262T Gene Polymorphism in the Susceptibility and Survival of Cancers. Sci Rep. 6: 26973. https://doi: 10.1038/srep26973
– Xu B, Sun J, Sun Y, Huang L, Tang Y, Yuan Y (2013). No evidence of decreased risk of colorectal adenomas with white meat, poultry, and fish intake: a meta-analysis of observational studies. Ann Epidemiol. 23(4):215-22. https://doi: 10.1016/j.annepidem.2012.12.016
– Yamagishi H, Kuroda H Imai Y, Hiraishi H (2016). Molecular pathogenesis of sporadic colorectal cancers. Chin J Cancer 35: 4. https://doi: 10.1186/s40880-015-0066-y
– Yang B, McCullough ML, Gapstur SM, Jacobs EJ, Bostick RM, Fedirko V et al. (2014). Calcium, vitamin D, dairy products, and mortality among colorectal cancer survivors: the Cancer Prevention Study-II Nutrition Cohort. J Clin Oncol 32(22):2335-43. https://doi: 10.1200/JCO.2014.55.3024
– Zinczuk J, Zaręba K, Kaminska J, Koper-Lenkiewicz OM, Dymicka-Piekarska V, Pryczynicz A et al. (2021). Association of tumour microenvironment with protein glycooxidation, DNA damage, and nitrosative stress in colorectal cancer, Cancer Manag. Res 13 : 6329–6348. https://doi: 10.2147/CMAR.S314940
