α-amylase inhibitory effect of several varieties of cheese: Gouda, Gruyere, Edam, Emmental

Authors

  • Houria Medjdoub Laboratory Antifungal, Antibiotic, Physico-chemical, Synthesis and Biological Activity, Department of Biology, Faculty of Natural Sciences and Life, Sciences of the Earth and the Universe, University Abou Bekr Belkaid Tlemcen 13000, BP 119, Algeria.
  • Waffa Bouali Laboratory Antifungal, Antibiotic, Physico-chemical, Synthesis and Biological Activity, Department of Biology, Faculty of Natural Sciences and Life, Sciences of the Earth and the Universe, University Abou Bekr Belkaid Tlemcen 13000, BP 119, Algeria.
  • Fatima Zohra Kebaha 2 Department of biology, Faculty of Natural Sciences and Life, Sciences of the Earth and the Universe, University Abou Bekr Belkaid Tlemcen 13000, BP 119, Algeria.
  • Manel Sedjai Department of Biology, Faculty of Natural Sciences and Life, Sciences of the Earth and the Universe, University Abou Bekr Belkaid Tlemcen 13000, BP 119, Algeria
  • Amane Ellah Kadri
  • Oumaima Senhadji Department of Biology, Faculty of Natural Sciences and Life, Sciences of the Earth and the Universe, University Abou Bekr Belkaid Tlemcen 13000, BP 119, Algeria

Keywords:

Cheese, Emmental, α-amylase inhibition, diabetes mellitus

Abstract

Introduction : Cheese is a highly nutritious fermented milk product with diverse flavor and texture. As a functional food it has gained a lot of attention in recent times because of their health benefitting properties.

Methods: In the present study, four varieties of cheese (Gouda, Gruyere, Edam and Emmental) are tested for their antidiabetic effect. In fact, an aqueous extract is prepared by maceration of cheese pieces in phosphate buffer. Inhibitory effect against α-amylase of extract, pH and proteins content are investigated.

Results : Results show pH value between 5.2 and 5.9 with protein content between 4.5 and 9.14%. Inhibitory effect against α-amylase is moderate with IC50 values of 49.29±4.02, 38.5±6.05, 70.66±12.52 and 25.9±6.82 mg.mL-1 for Goud, Gruyere, Edam and Emmental respectively.

Conclusion : In fact, we can conclude that Emmental cheese has the best inhibitory effect on porcine pancreatic α-amylase compared to Gouda, Gruyere and Edam cheeses.. Investigation of active ingredient in cheese especially in Emmental may be interesting.

References

– Ali MA, Kamal MM, Rahman MH, Siddiqui MN, Haque MA, Saha KK, Rahman MA (2022). Functional dairy products as a source of bioactive peptides and probiotics: Current trends and future prospectives. J Food Technol 59 (4):1263. 1279. https://doi.org/10.1007/s13197-021-05091-8

– Bezie A (2019). The effect of different heat treatment on the nutritional value of milk and milk products and shelf-life of milk products. A review. J. Dairy Vet Sci 11(5): 555822. https://doi.org/10.19080/JDVS.2019.11.555822

– De Sainte Marie C, Mariani M, Millet M, Cerdan C, Casabianca F (2020). Can raw milk cheese and pasteurised milk cheese coexist? Unthinkable or never really considered? Rev Agric Food Environ Studies 101:287-309. https://doi.org/10.1007/s41130-020-00106-y

– Genis DO, Bilge G, Sezer B, Durna S, Boyaci IH (2019). Identification of cow, buffalo, goat and ewe milk species in fermented dairy products using synchronous fluorescence spectroscopy. Food chem 284:60-66. https://doi.org/10.1007/s41130-020-00106-y

– Henry RJ, Cannon DC, Winkelman JW (1974). Clinical Chemistry-Principles and techniques. Harper et Row, 2nd Ed.

– Iwaniak A, Mogut D, Minkiewicz P, Żulewska J, Darewicz M (2022). An integrated approach to the analysis of antioxidative peptides derived from Gouda cheese with a modified β-casein content. Sci Rep 12(1):13314. https://doi.org/10.1038/s41598-022-17641-x

– Kumari VBC, Huligere SS, Alotaibi G, Al Mouslem AK, Bahauddin AA, Shivanandappa TB, Ramu R (2023). Antidiabetic activity of potential probiotics limosilactobacillus spp., levilactobacillus spp., and lacticaseibacillus spp. isolated from fermented sugarcane juice: a comprehensive in vitro and in silico study. Nutrients 15(8):1882. https://doi.org/10.3390/nu15081882

– Lala P. K. (1981). Practical Pharmacognosy. Calcutta, Lina Guha, 135.

– Liu H, Li Q, Zhao D, Zhang M, Jiang S, Li C (2021). Changes in the structure and digestibility of myoglobin treated with sodium chloride. Food Chem 363:130284. https://doi.org/10.1016/j.foodchem.2021.130284

– Mbye M, Sobti B, Al Nuami MK, Al Shamsi Y, Al Khateri L, Al Saedi R, Kamal-Eldin A (2020). Physicochemical properties, sensory quality, and coagulation behavior of camel versus bovine milk soft unripened cheeses. NFS journal 20:28-36. https://doi.org/10.1016/j.nfs.2020.06.003

– Miller GL (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426-429. https://doi.org/10.1021/ac60147a030

– Mladenović KG, Grujović MŽ, Kocić-Tanackov SD, Bulut S, Iličić M, Degenek J, Semedo-Lemsaddek T (2021). Serbian traditional goat cheese: physico-chemical, sensory, hygienic and safety characteristics. Microorganisms 10(1): 90. https://doi.org/10.3390/microorganisms10010090

– Moses D, Ginell GM, Holehouse AS, Sukenik S (2023). Intrinsically disordered regions are poised to act as sensors of cellular chemistry. Trends Biochem Sci 48 (12):1019-1034. https://doi.org/10.1016/j.tibs.2023.08.001

– Mushtaq M, Gani A, Noor N, Masoodi FA (2021). Phenotypic and probiotic characterization of isolated LAB from Himalayan cheese (Kradi/Kalari) and effect of simulated gastrointestinal digestion on its bioactivity. LWT 149 :111669. https://doi.org/10.1016/j.lwt.2021.111669

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Published

2024-04-30

How to Cite

Houria Medjdoub, Waffa Bouali, Fatima Zohra Kebaha, Manel Sedjai, Amane Ellah Kadri, & Oumaima Senhadji. (2024). α-amylase inhibitory effect of several varieties of cheese: Gouda, Gruyere, Edam, Emmental. Journal of Nutrition and Biotechnology Advancements, 1(01), 10–13. Retrieved from https://journals.univ-tlemcen.dz/JNBA/index.php/JNBA/article/view/6