Identification and pathogenicity of Fusarium spp. isolated from root and crown of rice in Golestan province

Abstract

In order to identify Fusarium spp. associated with root and crown’s rot of rice, several fields from different regions of Golestan province were sampled during growing season (2015-2018). especially, samples with yellowing and abnormal elongation, abnormal growth, decay root and foot. For isolation of fungi, discolored segments (root and crown) were surface disinfected with 0.5% NaOCl. Then cultured on Potato Dextrose Agar with pH. For exact observation some characteristics of species were used from Carnation Leaf Agar medium. Among the 30 Fusarium isolates, 6 species were identified as: Fusarium moniliforme*, F. fujikouri, F.verticiloides *, F. proliferatum*, F. concolor*, F.sacchari. on the basis of their morphological characteristics. Literature data showed that the Bakanae disease of rice all over the world is caused by F. moniliform and probably some other Fusarium species from section Liseola or allied. To investigate the possibility of using a part of bases TEF gene for differentiation of Fusarium species, some parts were amplified using two primers ef1 and ef2. The equivalent size was amplified of 700 bp However, from pathogenicity tests that have been carried out by using variety Khazar of rice it was evident that F.moniliform and F.proliferatum was highly virulent and the only species involved in causing Bakanae disease.