In silico study of the DNA sequences of the Coronavirus in several animal species and human
DOI:
https://doi.org/10.46325/gabj.v9i1.422Keywords:
Coronavirus, animals, humans, S gene, phylogenyAbstract
Coronaviruses are positive-sense single-stranded RNA viruses, and their name derives from their crown-like appearance. In this study, we inventoried the animal species affected by Coronaviruses, and then we performed a comparative phylogenetic analysis based on DNA sequences among the different viral strains of Coronaviruses that have affected these animals as well as humans. To carry out this work, we used MEGA software version 11 to analyze the DNA sequences extracted from GenBank of the S gene. We classified the viral strains of this gene according to the infected animal species and the geographical area in which they were found. We worked on 33 nucleotide sequences of the S gene from different strains, such as SARS-CoV-2 and MERS-CoV affecting humans, Bat-CoV affecting bats, and PEDV and PDCoV affecting pigs, across several countries including China, Saudi Arabia, the United States, Japan, and Spain. We found that the S gene is characterized by an accumulation of mutations among the different strains, with certain nucleotide changes that may consequently be responsible for the transmission of the virus from animals to humans. Moreover, our results show that the S gene exhibits a very high genetic diversity among the Coronavirus strains, whether they infect animals or humans in different countries.
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