First molecular detection and geographical distribution of Nosema apis & Nosema ceranae in indigenous honey bees reared in Algeria
DOI:
https://doi.org/10.46325/gabj.v7i2.343Keywords:
Geographical distribution, multiplex PCR, Apis mellifera intermissa, Nosema apis, Nosema ceranae, AlgeriaAbstract
The aim of the present study was to study with the molecular tools the presence and the geographical distribution of Nosema spp. in native honey bees reared in Algeria (Apis mellifera intermissa). The study was carried out on 51 samples of adult honey bees from 11 different regions of Algeria. Total DNA was extracted from the abdomen of honeybees, for each sample, pure genomic DNA was rehydrated in millipore filtered and deionized dH2O and stored at 4 °C. DNA samples were subjected to molecular detection by multiplex PCR using primers specific for a region of the 16S rRNA gene of the Nosema species. The results of the PCR show that Nosema apis (N. apis) and Nosema ceranae (N. ceranae) were detected in the bee samples examined. 22 samples (43.13%) were positive for N. ceranae of which three samples (5.88%) were positive as co-infection with N. apis and N. ceranae, while 29 samples (56.87%) are unscathed from nosemosis infection. Infection with N. apis has always appeared as a co-infection with N. ceranae and apiaries established in western Algeria. The climate is considered to be one of the main factors in the spread of Nosema species. This is the first report on detection of N. ceranae and N. apis, and the level of contamination and distribution pattern in Algeria. This work suggests that our A. mellifera populations may be genetically resistant to these substances, and nevertheless, these findings require further molecular genetics research to be confirmed
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