Antioxidant and cytotoxic activities of extracts from Haloxylon scoparium Pomel
DOI:
https://doi.org/10.46325/jnpra.v3i02.60Keywords:
Antioxidant activity; Cytotoxic activity; Haloxylon scoparium Pomel.Abstract
Haloxylon scoparium Pomel is a plant known to possess anti-cancer properties as well as anti-plasmodial and larvicidal activities. The aerial parts are used in traditional medicine to treat disorders and problems of the eye and vision, digestion problems, dermatoses, scorpion pitting. The antioxidant and anticancer activities of different extracts (dichloromethane, ethyl acetate and butanol) from flowers and stems were studied. The flowers exhibited strong antioxidant activity using the oxygen radical absorbance capacity method and a cell-based assay. The most interesting results are obtained with the ethyl acetate extracts of the flowers active with an ORAC value of 6.6 μmol TE / mg, followed by the butanol extract of the same organ (ORAC value of 6.22 μmol TE/ mg). The experiments carried out using the cellular antioxidant protocol reveal a very strong antioxidant activity, particularly for the ethyl acetate and butanol extract of the flowers with an IC50 value of 0.029 ± 0.004 µg/ mL and 0.3 ± 0.1 µg/ mL respectively . The cytotoxic activity (Resazurin tests) was found to be moderate against A-549 (lung carcinoma) and DLD-1(colon carcinoma) cells, with IC50 values of 110 ± 13 and 117 ± 14 μg/ mL, respectively. All the results obtained constitute a scientific justification for the use traditional of the plants studied and once again confirms the relevance of the remedies traditional in the treatment of many diseases.
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