Antioxidant and cytotoxic activities of extracts from Haloxylon scoparium Pomel


  • Samira BELHADJ TAHAR Laboratory of Biogeochemistry of desert environments Ouargla University, Faculty of Mathematics and Sciences of Matter, Ouargla, Algeria
  • André PICHETTE, Jean LEGAULT Laboratoire LASEVE, Université du Québec à Chicoutimi, 555, Boulevard de l’Université, Chicoutimi, Québec, Canada G7H 2B1
  • Mahfoud HADJ-MAHAMMED Laboratory of Biogeochemistry of desert environments Ouargla University, Faculty of Mathematics and Sciences of Matter, Ouargla, Algeria



Antioxidant activity; Cytotoxic activity; Haloxylon scoparium Pomel.


Haloxylon scoparium Pomel is a plant known to possess anti-cancer properties as well as anti-plasmodial and larvicidal activities. The aerial parts are used in traditional medicine to treat disorders and problems of the eye and vision, digestion problems, dermatoses, scorpion pitting. The antioxidant and anticancer activities of different extracts (dichloromethane, ethyl acetate and butanol) from flowers and stems were studied. The flowers exhibited strong antioxidant activity using the oxygen radical absorbance capacity method and a cell-based assay. The most interesting results are obtained with the ethyl acetate extracts of the flowers active with an ORAC value of 6.6 μmol TE / mg, followed by the butanol extract of the same organ (ORAC value of 6.22 μmol TE/ mg). The experiments carried out using the cellular antioxidant protocol reveal a very strong antioxidant activity, particularly for the ethyl acetate and butanol extract of the flowers with an IC50 value of 0.029 ± 0.004 µg/ mL and 0.3 ± 0.1 µg/ mL respectively . The cytotoxic activity (Resazurin tests) was found to be moderate against A-549 (lung carcinoma) and DLD-1(colon carcinoma) cells, with IC50 values of 110 ± 13 and 117 ± 14 μg/ mL, respectively. All the results obtained constitute a scientific justification for the use traditional of the plants studied and once again confirms the relevance of the remedies traditional in the treatment of many diseases.


Amiot, M, J., Fleuriet, A., & Macheix, J.J., (1886). Importance and evolution of phenolic compounds in olive during growth and maturation. Journal of Agricultural and Food Chemistry, 34 (5), 823-826.

Aruoma, Okezie I., Murcia, A., Butler, J., & Halliwell, B. (1993). Evaluation of the antioxidant and prooxidant actions of gallic acid and its derivatives. Journal of Agricultural and Food Chemistry, 41, (11), 1880-1885.

Bakchiche, B., Gherib, A., Aazzab, S., Custódia Gagob., Grac, M., & Miguel, A. (2013). Antioxidant activities of eight Algerian plant extracts and two essential oils. Industrial Crops and Products 46, 85–96.

Benkrief, R., Brum-Bousquet, M. Tillequin, F., Koch, M. (1990). Alkaloids and flavonoid from aerial parts of Hammada articulata ssp. Scoparia. Annales Pharmaceutiques francaises 48, (4). 219-224.

Boxin, Ou ., Maureen Hampsch-Woodill., and Ronald L. Prior., (2001). Development and validation of an improved oxygen radical absorbance capacity assay usin fluorescein as the fluorescent probe. Journal of Agricultural and Food Chemistry, 49(10), 4619-4626.

Dávalos, A., Gómez-Cordovés, C. & Bartolome. (2004). Extending applicability of the oxygen radical absorbance capacity (ORAC-Fluorescein) assay. Journal of Agricultural and Food Chemistry, 52, (48–54), 1520-5118.

Ehlenfeldt, K., & Prior, R.L. (2001). Oxygen radical absorbance capacity (ORAC) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry. Journal of Agricultura land Food Chemistry, 49, 2222–2227.

Elisia, I., H.U, C., Popovich, D.G., & Kitts, D.D. (2007). Antioxidant assessment of ananthocyanin- enriched blackberry extract. Food Chemistry, 101, 1052–1058.

Farnsworth, N.R. (1966). Biological and phytochemical screening of plants. Journal of Pharmaceutical Sciences, 55, 225-276.

Frankle, E.N. (2005). Lipid Oxidation, The Oily Press, Bridgwater, English, p.470.

Girard-Lalancette, K K., Pichette, A., & Legault, J. (2009). Sensitive cell-based assay using DCFH oxidation for the determination of pro-and antioxidant properties of compounds and mixtures: Analysis of fruit and vegetable juices. Food Chemistry,115, 720–726.

Huang, D., Boxin, Ou., Hampsch-Woodill, M., Flanagan, J., A., & Prior, R.L. (2002). High-throughput assay of oxygen radical absorbance capacity (ORAC) using amultichannel liquid handling system coupled with a microplate fluorescence readerin96-Wellformat. Journal of Agricultural and Food Chemistry, 50, 4437–4444.

Iserin, P., Masson, M., Restellini, J. P., Ybert, E., De Laage de Meux, A., Moulard, F., Zha, E., De la Roque, R., De la Roque, O., Vican, P., Deelesalle –Féat, T., Biaujeaud, M., Ringuet, J., Bloth J., & Botrel, A. (2001). Larousse des plantes médicinales : identification, préparation, soins. Ed Larousse.10-12.

Islam, B.U.; Suhail, M.; Khan, M.K.; Zughaibi, T.A.; Alserihi, R.F.; Zaidi, S.K.; Tabrez, S.

(2020). Polyphenols as anticancer agents: Toxicological concern to healthy cells. Phytother. Res., 35, 6063–6079. doi: 10.1002/ptr.7216.

Negi, P.S., Chauhan, A.S., Sadia, G.A., Rohinishree, Y.S., & Ramteke, R.S. (2005). Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts. Food Chemistry, 92, 119–124.

Newman, D.J., Cragg, G, M., & Snader, K.M. (2003). Natural products as sources of new drugs over the period 1981–2002. Journal of Natural Products, 66 (7): 1022– 1037.

O’brien, J., Wilson, I., Orton,T., & Pognan, F. (2000). Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity: Resazurin as a cytotoxicity assay. European Journal of Biochemistry, 267, 5421–5426.

Oshima, H., Yoshie, Y., Auriol, S., & Gilibert, I. (1998). Antioxidant and pro-oxidant actions of flavonoids: effects on DNA damage induced by nitric oxide, peroxynitrite and nitroxyl anion. Free Radical Biology and Medicine, 25(9), 1057-1065

Oueslati, S., Ksouri, R., Falleh, H., Pichette, A., Abdelly, C., & Legault, J. (2012). Phenolic content, antioxydant, anti-inflammatory, and anti-cancer activities of the edible halophyte Suaeda fruticosa Forssk. Journal of Food Chemistry, 132, 943-947.

Pandey, K., & Rizvi., S.I. (2009). Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity, 2(5), 270-278.

Quezel, P., Santa S. (1963). Nouvelle flore de l’Algérie et des régions désertiques méridionales. Tome II, Ed. CNRS, Paris.

Saura, S.C., Gray, G.C. (1996). Pro-oxidant activity of flavonoids: effects on glutathione and glutathione S-transferase in isolated rat liver nuclei. Cancer Letters, 104 (2), 193-196. 10.1016/0304-3835(96)04251-6

Shashi B, Jaswant S, Madhusudana RJ, Kumar SA, Nabi QG(2006) . A novel lignan compo sition from Cedrus deodara induces apoptosis and early nitric oxide generation in humanleukemia Molt-4 and HL-60 cells. Nitric Oxide.;14(1), 72-88.

doi: 10.1016/j.niox.2005.09.009.

Small, E., & Catling, P.M. (2000). Les cultures médicinales canadiennes. Presses scientifiques du CNRC, Ottawa (Ontario), Canada. 281. ISBN 0-660-96380-9.NRC No. 42253.

Spiteller, G. (2006). Peroxyl radicals: Inductorsof neurodegenerative and other inflammatory

diseases. Their origin and how they transform cholesterol, phospholipids, plasmalogens,

polyunsaturated fatty acids, sugars and proteins into deleterious products. Free Radical

Biology and Medicine.41, 362–387.

W.-C. Zeng a b, Qiang He a, Qun Sun c, Kai Zhong a, Hong Gao. (2012). Antibacterial activity of water-soluble extract from pine needles of Cedrus deodara. International Journal of Food Microbiology.153 (1–2),78-84.




How to Cite

Samira BELHADJ TAHAR, André PICHETTE, Jean LEGAULT, & Mahfoud HADJ-MAHAMMED. (2024). Antioxidant and cytotoxic activities of extracts from Haloxylon scoparium Pomel. Journal of Natural Product Research and Applications, 3(02), 1–10.