Phycochemistry study and antimicrobial activity of Spirogyra freshwater green microalgae from Algeria
DOI:
https://doi.org/10.46325/jnpra.v3i02.65Keywords:
Oued El Mellah; algae; Spirogyra; phycochemical componds; antimicrobial activityAbstract
In Algeria, algae have not been adequately explored for their potential as a source of bioactive substances. In this context the blooms of green alga Spirogyra sp. were collected from freshwater habitats in Oued El Mellah near M’sila province of Algeria. Hexane, acetone and ethanol crude extracts were screened for antimicrobial activity against Staphylococcus aureus, Listeria monocytogenes, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Candida albicans. The contents of total soluble sugars, soluble proteins, total lipids and phenolic acids were also determined. The present findings revealed that the highest extraction yield was obtained from ethanol (4.93%) compared to acetone (3.67%) and hexane (2.33%). The contents of phycochemical compounds were 32.5% of soluble carbohydrates, 15.84% of lipids, 15.6% of phenolic acids and 5.16% of proteins. All extracts exhibited better and stronger antibacterial activities against the five pathogenic bacteria with inhibition zones diameter ranged from 13.5-29 mm for acetone extract, from 13-25 mm for ethanol extract and from 12.5-20.5 mm for hexane extract at 7.5 µL. The minimal inhibitory concentration (MIC) was in the range of 15-40 mg mL-1. The acetone extract was more effective than others extracts with MIC value of 15 mg mL-1 for all bacteria. These findings indicate the presence of promising antibacterial compounds originating from microalgae, which can be exploited for the production of new antimicrobial agents.
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