Antioxidant Activity of Brown Seaweed (Padina pavonica (L.) Extracts From the Algerian Mediterranean Coast

Abstract

The crude methanolic extract, ethyl acetate and butanolic fractions, tannins, saponins and polysaccharides of the marine brown alga, Padina pavonica, growing on the Algerian Mediterranean Coast were measured for antioxidant activity, using total antioxidant capacity (TAC), reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. Total phenolic, flavonoid and proanthocyanidin contents were determined. The total phenolic, proanthocyanidin and flavonoid contents of methanolic extract were about of 2.007 ± 0.104 mg GAE/g DM, 4.611 ± 0.346 mg CE/g DM and 1.132 ± 0.091 mg CE/g DM, respectively. The total antioxidant capacity was higher (4.365 ± 0.452 mg AAE/g DM) in crude methanolic extract. For the other two tests, the tannins and ethyl acetate fraction presented high activities compared to the other extracts with EC50 about 1.430 ± 0.000 and 1.615 ± 0.009 mg/mL), respectively for the reducing power and 5.718 ± 1.111 and 5.848 ± 0.186 mg/mL for the DPPH radical scavenging. The seaweed extracts displayed moderate antioxidant activity compared to ascorbic acid.

REFERENCES:

Aidi Wannes, W., Mhamdi, B., Sriti, J., Ben Jemia, M., Ouchikh, O., Hamdaoui, G., Kchouk,
M.E., & Marzouk, B. (2010). Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf stem and flower. Food and
Chemistry Toxicology, 48 (5), 1362–1370.
Applebaum, S.W., Marco, S., & Birk, Y. (1969). Saponins as possible factors of resistance of
legume seeds to the attack of insects. Journal of Agricultural and Food Chemistry, 17
(3), 618–622.
Bekkara, F., Jay, M., Viricel, M.R., & Rome, S. (1998). Distribution of phenolic compounds
within seed and seedlings of tow Vicia faba cvs differing in their seed tannin content,
and study of to their seed and root phenolic exudation. Plant and Soil, 203 (1), 27–36.
Ben Said, R., El Abed, A., & Romdhane, M. S. (2002). Etude d’une Population de l’algue
brune Padina pavonica (L) Lamouroux A Cap Zebib (Nord De La Tunisie). Bulletin de
l'Institut National Scientifique et Technique d'Oceanographie et de Peche de
Salammbo, 29, 95–103.
Chbani, A., Mawlawi, H., & Eltahiri, S. (2011). Antibacterial activity of brown seaweed
extracts of Padina pavonica from the Mediterranean coast of Lebanon. Phytothérapie,
9, 283–286.
Chbani, A., Mawlawi, H., & Zaouk, L. (2013). Evaluation of brown seaweed (Padina
pavonica) as biostimulant of plant growth and development. African Journal of
Agricultural Research, 8 (13), 1155–1165.
Demirel, Z., Yildirim, Z.D., Tuney, I., Kesici, K., & Sukatar, A. (2012). Biochemical analysis
of some brown seaweeds from Aegean Sea. Botanica Serbica, 36, 91-95.
Fischer, W., Scheider, M., & Bauchot, M.L. (1987). Fiches FAO d'identification des espèces
pour les besoins de la pêche. Méditerranée et Mer noire; Zone de pêche 37. [Species
identification sheets FAO for fishery purposes. Mediterranean and black sea; fishing area
37].Vol.1. Food and Agriculture Organisation of the United Nations, Rome. p. 758.
Indu, H., & Seenivasan, R. (2013). In vitro antioxidant activity of selected seaweeds from
southeast coast of India, International Journal of Pharmacy and Pharmaceutical
Sciences, 5(2), 474–484.
Ismail-Ben Ali, A., Ktari, L., Boudabbous, A., & El Bour, M. (2010). Seasonal variation of
antibacterial activity of the brown alga Padina pavonica (L) thivy collected from
Northern coast of Tunisia. Bulletin de l'Institut National Scientifique et Technique
d'Oceanographie et de Peche de Salammbo, 37, 111–116.
Julkunen-Titto, R. (1985). Phenolic constituents in the leaves of northern willows: methods
for the analysis of certain phenolics. Journal of Agricultural and Food Chemistry, 33
(2), 213–217.
Kamenarska, Z., Gasic, M. J., Zlatovi, M., Razovic, A., Sladic, D., Kljajic, Z., Stefanov, K.,
Seizova, K., Najdenski, H., Kujumgieva, A., Tsvetkova, I., & Popo, S. (2002).
Chemical composition of the brown Alga Padina pavonia (L.) Gaill. from the Adriatic
Sea. Botanica Marina, 45, 339–345.
Kokabi, M., Yousefzadi, M., Ali ahmadi, A., Feghhi, M.A., & Keshavarz, M. (2013).
Antioxidant Activity of Extracts of Selected Algae from the Persian Gulf, Iran. Journal
of the Persian Gulf (Marine Science), 4(6), 45–50.
Ksouri, J., Elferjani, H., & Mensi, F. (2008). Estimation du stock naturel de l’algue brune
Padina pavonica (L.) Thivy en Tunisie septentrionale (Cap Zebib). Bulletin de l'Institut
National Scientifique et Technique d'Oceanographie et de Peche de Salammbo, 35, 57–
60.
Ktari, L., & Guyot, M. (1999). A cytotoxic oxysterol from the marine alga Padina pavonica
(L.) Thivy. Journal of Applied Phycology, 11 (6), 511–513.
Ktari, L., Longeon, A., & Guyot, M. (2001). Biological activities of marine algae from
Tunisian coasts. Journal de Recherche Océanographique, 26, 37–41.

Kumar Chandini, S., Ganesan, P., & Bhaskar, N. (2008). In vitro antioxidant activities of
three selected brown seaweeds. Food Chemistry, 107, 707–713.
Naja, K., Mawlawi, H., & Chbani, A. (2012). Antioxidant and Antifungal activities of Padina
Pavonica and SargassumVulgarefrom the Lebanese Mediterranean Coast. Advances in
Environmental Biology, 6(1), 42–48.
Omezzine, F., Haouala, R., El Ayeb, A., & Boughanmi, N. (2009). Allelopathic and
antifungal potentialities of Padina pavonica (L.) extract. Journal of Plant Breeding and
Crop Science, 1(4), 194–203.
Oyaizu, M. (1986). Studies one products of browning reaction prepared from glucose amine.
Japanese Journal of Nutrition, 44 (6), 307–315.
Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant
capacity through the formation of a phosphomolybdenum complex: specific application
to the determination of vitamin E. Analytical Biochemistry, 269 (2), 337–341.
Sanchez-Moreno, C., Larrauri, J.A., & Saura-Calixto, F. (1998). A procedure to measure the
antiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture,
76 (2), 270–276.
Sari, A., & Tuzen, M. (2009). Equilibrium, thermodynamic and kinetic studies on aluminum
biosorption from aqueous solution by brown algae (Padina pavonica) biomass.
Journal of Hazardous Materials, 171, 973–979
Singleton, C.P., & Rossi, J.A. (1965). Colorimetry of Total Phenolics with
Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enolology and
Viticulture, 16 (3), 144–158.
Teodosio Melo, K.R., Gomes Camara., R.B., Queiroz, M.F., Jacome Vidal, A.A., Machado
Lima, K.R., Melo-Silveira, R.F., Almeida-Lima, J., & Oliveira Rocha, H.A. (2013).
Evaluation of Sulfated Polysaccharides from the Brown Seaweed Dictyopteris Justii as
Antioxidant Agents and as .Inhibitors of the Formation of Calcium Oxalate Crystals.
Molecules, 18, 14543–14563
Wijesekara, I., Pangestutia, R., & Kima, S.K. (2011). Biological activities and potential
health benefits of sulfated polysaccharides derived from marine algae. Carbohydrate
Polymers, 84, 14–21
Zhang, S.Y., Zheng, C.G., Yan, X.Y., & Tian, W.X. (2008). Low concentration of condensed
tannins from catechu significantly inhibits fatty acid synthase and growth of MCF-7
concealments. Biochemical and Biophysical Research Communications, 371 (4), 654–
658.
Zhenfei, P., Min, L., Zhexiang, F., Jiulin, W., & Qiqing Z. (2012). Composition and
cytotoxicity of a novel polysaccharide from brown alga (Laminaria japonica).
Carbohydrate Polymers, 89, 1022– 1026.
Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid content
in mulberry and to their scanenging effects one superoxide radicals. Food Chemistry,
64 (4), 555–559.