Beyond the Centrifuge: A Systematic Review of Automated and Microfluidic DNA Extraction Innovations

Authors

  • Dr Farah Djelti CancerLab, Faculty of Medical Sciences, University of Tlemcen, Tlemcen, Algeria
  • Pr. Tarik M. Chaouche Natural Products Laboratory, Department of Biology, Aboubekr Belkaïd University,
  • Dr. Moussa BELHADJ
  • Dr. Sarah LAMARI
  • Dr. Meknassia DEBBAL
  • Dr. Imane GHELAI
  • Dr. Houssam BOULANOIR

DOI:

https://doi.org/10.46325/f42rzd14

Keywords:

DNA Extraction, Microfluidics, Systematic Review

Abstract

This systematic review following PRISMA standards evaluates DNA extraction methods published in scientific journals over the past ten years. Results show that silica column-based purification continues to be the most popular technique in molecular biology labs, consistently producing high amounts of genomic DNA with excellent purity levels. At the same time, magnetic bead separation technology is becoming increasingly common in automated, large-scale operations, though DNA yields are generally lower compared to column methods.

The cost comparison shows clear differences: automated robotic systems require much higher expenses per sample than traditional manual CTAB (cetyltrimethylammonium bromide) extraction. However, these automated platforms provide better consistency and more reliable results between experiments. When combining data from multiple studies, we found significant differences in how well various methods extract DNA, with particular problems in recovering viral RNA. These difficulties are especially severe when samples contain PCR inhibitors substances like humic acids, polysaccharides, and bile salts, commonly found in challenging samples such as soil and fecal material. 

These findings highlight the strong need for standardized DNA extraction protocols across laboratories. Future improvements could include using artificial intelligence to optimize extraction procedures and developing microfluidic chip-based systems to reduce errors and improve DNA recovery from difficult biological samples.

Author Biographies

  • Dr Farah Djelti, CancerLab, Faculty of Medical Sciences, University of Tlemcen, Tlemcen, Algeria

    Biology

  • Pr. Tarik M. Chaouche, Natural Products Laboratory, Department of Biology, Aboubekr Belkaïd University,

    biochemistry

  • Dr. Moussa BELHADJ

    biology

  • Dr. Sarah LAMARI

    biology

  • Dr. Meknassia DEBBAL

    biochemistry

  • Dr. Houssam BOULANOIR

    genetic

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01/20/2026

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Vol. 10 No. 1 (2026): V10.N1.2026

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Review Article

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Beyond the Centrifuge: A Systematic Review of Automated and Microfluidic DNA Extraction Innovations. (2026). Genetics & Biodiversity Journal , 10(1), 74-91. https://doi.org/10.46325/f42rzd14

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