Assessment of in vitro antioxidant and in vivo anti-inflammatory activities of marine algae from Algerian coast
DOI:
https://doi.org/10.46325/jnpra.v2i1.40Keywords:
Sargassum vulgare, Cladostephus hirsutus, Rissoella verruculosa, Antioxidant activity, acute toxicity, anti-inflammatory activity.Abstract
The search for natural compounds with pharmaceutical activity indicated marine macroalgae as promising sources to supply novel compounds with potential bioactivities. In this study, three algae from the northwest coast of Algeria Sargassum vulgare, Cladostephus hirsutus and Rissoella verruculosa were investigated for their in vitro antioxidant activity and for their in vivo anti-inflammatory activity. Total phenolic, total flavonoid and flavonol, and condensed tannin contents were determined in crude extracts and petroleum ether, ethyl acetate (EA), dichloromethane and butanol fractions, and aqueous residues. Antioxidant activity was evaluated using several in vitro assays: Total Antioxidant Capacity, Ferric Reducing Antioxidant Power and diphenyl picryl hydrazyl radical scavenging activity. Acute toxicity of the crude extract of C. hirsutus was studied in Swiss albino mice and anti-inflammatory activity was evaluated using carrageenan-induced paw edema. Crude extracts revealed high total antioxidant activity ranging from 1.27 to 1.63 mg ascorbic acid equivalent/gram of dry matter for R. verruculosa and C. hirsutus, respectively. For the three algae, EA fractions showed the highest antioxidant activity by the three assays tested, with up to 67% of DPPH radical scavenging activity and an EC50 of 1.114 mg ml-1 by the ferric reducing power assay for the EA fraction of R. verruculosa. No signs of acute toxicity were observed in the crude extract of C. hirsutus at 150 and 300 mg/kg. Significant and dose-dependent activity was shown against acute inflammation with edema reduction of 58.86% and 71.01% at 150 and 300 mg/kg, respectively, after 6 hours of carrageenan administration.
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